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BCA Protein Assay Kit

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MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.

  • Storage :
    RT, 1 year. After preparing the protein standard solution, please store it at -20ºC.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.

The detection principle is as follows: under alkaline conditions, proteins reduce Cu2+ to Cu+, and the resulting Cu+ forms a purple-colored complex with BCA molecules. This complex exhibits a strong absorbance at 562 nm, with the absorbance showing a linear correlation with the protein concentration in the sample. This product enables accurate quantification of protein concentrations in the range of 0-2000 µg/mL.

Protocol

For accurate and reliable results, please refer to the manual for detailed experimental procedures.

1. Preparation of BSA Standards

Prepare the protein standard solution using the supplied BSA and generate a series of standards according to the expected sample concentration range. A fresh standard curve is recommended for each protein assay.

Standard BSA concentration (μg/mL)
S0 0
S1 125
S2 250
S3 500
S4 1000
S5 1500
S6 2000

2. Preparation of BCA Working Reagent

Prepare the required amount of BCA working reagent by mixing Reagent A and Reagent B at a volume ratio of 50 : 1. Mix thoroughly before use. Prepare approximately 5%-10% excess working reagent according to the number of wells.

3. 96-Well Plate Method

Component 96-well plate method
BSA standard or test sample 25 μL
BCA working reagent 200 μL

1. Add BSA standards and protein samples to a 96-well plate. Replicate wells are recommended.

2. Add 200 μL freshly prepared BCA working reagent to each well and mix gently.

3. Incubate at 37°C for 30 min.

4. Cool to room temperature and measure absorbance at 562 nm.

5. Plot BSA concentration versus A562, calculate sample protein concentration from the standard curve, and multiply by the corresponding dilution factor.

Note: The quantitative range of the kit is 0-2000 μg/mL. If samples contain high concentrations of interfering substances, dilution or dialysis is recommended.

Storage

RT, 1 year.

After preparing the protein standard solution, please store it at -20ºC.

Attention

1. It is recommended to prepare a new standard curve for each protein assay to ensure the accuracy and reproducibility of the results.

2. If the sample contains high concentrations of interfering substances, dilution or dialysis is recommended. If accurate results still cannot be obtained, alternative protein quantification methods—such as the Bradford Protein Assay Kit (Cat. No.: HY-K2001)—can be used.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.

Components

Components HY-K2002-500 T HY-K2002-2000 T
Reagent A 100 mL 400 mL
Reagent B 2.5 mL 10 mL
BSA 30 mg 30 mg × 2

Documentation

MOQ
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100 mg

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