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Kindlin 2 Antibody (YA6969)

(Synonyms: FLJ34213 antibody; FLJ44462 antibody; UNC112 antibody; FERM2_HUMAN antibody; Fermitin family homolog 2 antibody; Fermt2 antibody; KIND2 antibody; Kindlin-2 antibody; MIG-2 antibody; MIG2 antibody)
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Based on 1 Customer Validation

Kindlin 2 Antibody (YA6969) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kindlin 2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Kindlin 2 Antibody (YA6969)
  • Kindlin 2 Antibody (YA6969) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:100 1-2μg/sample

Product Details

Description

Kindlin 2 Antibody (YA6969) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kindlin 2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Immunogen

Synthetic peptide within human Kindlin 2 aa 301-350 / 680.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Kindlin 2 Antibody (YA6969)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using Kindlin 2 Antibody (HY-P87286) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Kindlin 2 is a Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling

  • Subcellular Localization

    Cytoplasm,Cytoplasm, cell cortex,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, stress fiber,Cell junction, focal adhesion,Membrane,Cell projection, lamellipodium membrane,Nucleus,Cytoplasm, myofibril, sarcomere, I band,Cell surface

  • Expression


    Tissue_Specificity: Ubiquitous. Found in numerous tumor tissues
    Induction: By serum in lung fetal fibroblast cultured cells

  • Isoforms & Post-Translational Modification

    Q96AC1 has three isomers: Q96AC1-1: 77861 Da (predicted); Q96AC1-2: 72397 Da (predicted); Q96AC1-3: 78675 Da (predicted).

  • Subunit

    Interacts with ILK (By similarity)

  • SwissProt ID

    Q96AC1

  • Synonyms

    FLJ34213 antibody; FLJ44462 antibody; UNC112 antibody; FERM2_HUMAN antibody; Fermitin family homolog 2 antibody; Fermt2 antibody; KIND2 antibody; Kindlin-2 antibody; MIG-2 antibody; MIG2 antibody

Kindlin 2 Antibody (YA6969) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using Kindlin 2 Antibody (HY-P87286) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Kindlin 2 Antibody (YA6969) Product Image
    1/16
  • Experimental Validation Results for Kindlin 2 Antibody (YA6969)
  • Kindlin 2 Antibody (YA6969) Product Image
  • Experimental Validation Results for Kindlin 2 Antibody (YA6969)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using Kindlin 2 Antibody (HY-P87286) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Kindlin 2 Antibody (YA6969)
  • Kindlin 2 Antibody (YA6969) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using Kindlin 2 Antibody (HY-P87286) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Kindlin 2 Antibody (YA6969) Product Image WB