Kindlin 2 Antibody (YA6969)

Cat. No.: HY-P87286: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Kindlin 2 Antibody (YA6969) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kindlin 2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Kindlin 2 Antibody (YA6969) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Kindlin 2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 78 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96AC1

Immunogen

Synthetic peptide within human Kindlin 2 aa 301-350 / 680.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IP IP: Immunoprecipitation	1-2μg/sample

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse heart (lane 3(20μg)) and Rat heart (lane 4(20μg)) using Kindlin 2 Antibody (HY-P87286) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling

Subcellular Localization：Cytoplasm,Cytoplasm, cell cortex,Cytoplasm, cytoskeleton,Cytoplasm, cytoskeleton, stress fiber,Cell junction, focal adhesion,Membrane,Cell projection, lamellipodium membrane,Nucleus,Cytoplasm, myofibril, sarcomere, I band,Cell surface

Expression：
Tissue_Specificity: Ubiquitous. Found in numerous tumor tissues
Induction: By serum in lung fetal fibroblast cultured cells

Isoforms & Post-Translational Modification：Q96AC1 has three isomers: Q96AC1-1: 77861 Da (predicted); Q96AC1-2: 72397 Da (predicted); Q96AC1-3: 78675 Da (predicted).

Subunit：Interacts with ILK (By similarity)

Synonyms

FLJ34213 antibody; FLJ44462 antibody; UNC112 antibody; FERM2_HUMAN antibody; Fermitin family homolog 2 antibody; Fermt2 antibody; KIND2 antibody; Kindlin-2 antibody; MIG-2 antibody; MIG2 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)