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  6. Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO)

Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) 

Cat. No.: HY-K0012
Manual SDS

MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.

Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO)
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 24 In-stock
10 mL USD 165 In-stock
50 mL USD 662 In-stock
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    Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Jun;26(6):962-974.  [Abstract]

    Western blot showing protein levels of TCF3 and TCF12 in WT and Tcf3/12 DKO GO-P5 ovaries (n=3 biological replicates).

    • Description

    • Storage

    • Protocol

    • Componments

    • Documentation

    Description
    & Advantages

    MCE Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) protects proteins from degradation by endogenous proteases released during protein extraction and purification.

    This cocktail is supplied as a ready-to-use solution in DMSO. The individual components of this protease inhibitor cocktail have specific inhibitory properties. AEBSF inhibits serine proteases, including trypsin, chymotrypsin, plasmin, kallikrein, and thrombin. Bestatin inhibits aminopeptidases, such as leucine aminopeptidase and alanyl aminopeptidase. E-64 acts against cysteine proteases, such as calpain, papain, cathepsin B, and cathepsin L. Pepstatin A inhibits aspartic proteases, such as pepsin (human or porcine), renin, cathepsin D, chymosin (bovine rennin), and protease B (Aspergillus niger).

    Storage

    -20°C, 2 years
    Protocol

    1.    This product can be applied in Western Blot analysis, Co-IP, pull-down,IF, IHC, kinase assay and etc.

    2.    Thaw at room temperature, add at 1:100 (v/v) dilution to 1 solution samples, mix throughly. It is recommended to prepare solutions of this product fresh when needed.

    3.    If necessary, 0.1 M EDTA can be prepared and added to the mix by the ratio of 1:100 (v/v).
    Componments
    Cat. No. Product Name Package
    HY-K0012-1 mL Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) 1 mL
    HY-K0012-10 mL Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) 1 mL × 10
    HY-K0012-50 mL Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO) 1 mL × 50
    Documentation
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Protease Inhibitor Cocktail, Bacteria (EDTA-Free, 100× in DMSO)
    Cat. No.:
    HY-K0012
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