Advanced MEM Reduced Serum Medium 

Minimum Essential Medium (MEM), also known as Minimal Essential Medium, was developed by Harry Eagle based on Eagle’s Basal Medium (BME). It is a basic cell culture medium with a simple formulation and broad applicability, and is one of the most commonly used media in animal cell culture.

MCE Advanced MEM Reduced Serum Medium is a chemically defined essential medium developed based on classical MEM formulation. Enables at least a 50% reduction in fetal bovine serum (FBS) usage while maintaining normal cell growth rates and cellular morphology, and is suitable for the culture of a wide variety of suspension and adherent mammalian cells.

The product is suitable for routine reduced-serum cell culture applications and can also be used as a transfection medium.

MCE offers various formulations of MEM medium to meet the diverse requirements of different cell culture applications.

4°C, 1 year

Protect from light.

For Cell Transfection

Follow standard transfection procedures and supplement the medium with fetal bovine serum (FBS) at 50% of the conventional concentration. Replace the culture medium with this formulation prior to transfection, and perform a medium change again 6–12 h after transfection.

For Routine Cell Culture

Supplement the medium with 2–5% fetal bovine serum (FBS). The optimal FBS concentration may be adjusted for individual cell lines to minimize serum usage whenever possible.

Note: MEM employs a sodium bicarbonate buffering system, it requires a 5–10% CO₂ atmosphere to maintain physiological pH stability.

1. This product has been sterile-filtered. Pay attention to aseptic operation to avoid contamination.

2. This product is for R&D use only, not for drug, household, or other uses.

3. For your safety and health, please wear a lab coat and disposable gloves to operate.