Blasticidin S, Sterile
Based on 5 publication(s) in Google Scholar
MCE Blasticidin S, Sterile (10 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation.
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Storage :
-20°C; 2 years.
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Description & Advantages
MCE Blasticidin S, Sterile (10 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It can be used as a selection agent in both mammalian cells and bacterial cells.
Resistance to Blasticidin S is conferred by bsr and BSD. Therefore, Blasticidin S is a popular and effective antibiotic for selecting vectors bearing the bsr or BSD gene in mammalian cell lines. The recommended working concentration ranges from 1 to 20 μg/mL depending on the cell line and 50-100 μg/mL for bacterial selection. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S.
Protocol
The Blasticidin S solution can be used according to the following methods:
1. Add Blasticidin S solution in proportion to the sterile cell culture solution, mix well before use.
2. Add Blasticidin S solution in proportion to the cell culture solution (the cell culture medium contains bacteria), filter and sterilize.
Publications
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Journal Impact Factor
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Most Recent
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Mol CellIntrinsic bias of the genetic code shapes the folding and stability landscapes of microproteins. [Abstract]2026 Jun 4;86(11):2173-2190.e11. PMID: 42161277
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Cell Rep MedA combined "eat me/don't eat me" strategy based on exosome for acute liver injury treatment. [Abstract]2025 Apr 15;6(4):102033. PMID: 40120577
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Cell RepSEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. [Abstract]2026 Apr 25;45(5):117297. PMID: 42035418
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J EndocrinolFat's all, folks: culturing and manipulating peri-prostatic adipocytes to probe impacts on prostate cancer biology. [Abstract]2026 Jan 23;268(1):e250256. PMID: 41524592
Storage
-20°C; 2 years.
Components
| Components | HY-K1054-1 mL | HY-K1054-5 mL |
|---|---|---|
| Blasticidin S, Sterile (10 mg/mL) | 1 mL | 5 mL |
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