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  2. Cell Biology
  3. Cell Culture
  4. Sterile Antibiotic Solution
  5. Blasticidin S, Sterile

Blasticidin S, Sterile 

Cat. No.: HY-K1054
Manual SDS

MCE Blasticidin S, Sterile is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation.

Blasticidin S, Sterile
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 78 In-stock
Estimated Time of Arrival: December 31
5 mL USD 312 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It can be used as a selection agent in both mammalian cells and bacterial cells.

Resistance to Blasticidin S is conferred by bsr and BSD. Therefore, Blasticidin S is a popular and effective antibiotic for selecting vectors bearing the bsr or BSD gene in mammalian cell lines. The recommended working concentration ranges from 1 to 20 μg/mL depending on the cell line and 50–100 μg/mL for bacterial selection. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S.

Storage

-20°C; 2 years.

Protocol

The Blasticidin S solution can be used according to the following methods:

1.   Add Blasticidin S solution in proportion to the sterile cell culture solution, mix well before use.

2.   Add Blasticidin S solution in proportion to the cell culture solution, filter and sterilize.

Components
Components HY-K1054-1 mL HY-K1054-5 mL
Blasticidin S, Sterile (10 mg/mL) 1 mL 5 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Blasticidin S, Sterile
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HY-K1054
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