1. Anti-infection
  2. Bacterial

Puromycin Dihydrochloride (Synonyms: CL13900 dihydrochloride)

Cat. No.: HY-B1743A Purity: 99.63%
Handling Instructions

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic isolated from the bacterium Streptomyces alboniger.

For research use only. We do not sell to patients.
Puromycin Dihydrochloride Chemical Structure

Puromycin Dihydrochloride Chemical Structure

CAS No. : 58-58-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
10 mg USD 60 In-stock
50 mg USD 192 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Other Forms of Puromycin Dihydrochloride:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic isolated from the bacterium Streptomyces alboniger.

In Vitro

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1]. Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2]. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.8368 mL 9.1839 mL 18.3678 mL
5 mM 0.3674 mL 1.8368 mL 3.6736 mL
10 mM 0.1837 mL 0.9184 mL 1.8368 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

544.43

Formula

C₂₂H₃₁Cl₂N₇O₅

CAS No.

58-58-2

SMILES

OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 10 mM

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.63%

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Puromycin Dihydrochloride
Cat. No.:
HY-B1743A
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