1. Anti-infection
  2. Bacterial

Puromycin Dihydrochloride (Synonyms: CL13900 dihydrochloride)

Cat. No.: HY-B1743A Purity: 99.86%
Handling Instructions

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic isolated from the bacterium Streptomyces alboniger.

For research use only. We do not sell to patients.

Puromycin Dihydrochloride Chemical Structure

Puromycin Dihydrochloride Chemical Structure

CAS No. : 58-58-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
50 mg USD 140 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Other Forms of Puromycin Dihydrochloride:

    Puromycin Dihydrochloride purchased from MCE. Usage Cited in: ROYAL SOCIETY OPEN SCIENCE. 29 August 2018.

    HEKs are transfected with CRISPR/CAS9-gRNA for miR-143 or miR-145 for 48 h and then treated with nutlin-3a and Puromycin for another 24 h. The protein expression of NRAS and target genes in the PI3 K/ATK pathway is analysed by western blotting.
    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic isolated from the bacterium Streptomyces alboniger.

    In Vitro

    Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1]. Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2]. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 77.5 mg/mL (142.35 mM)

    H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8368 mL 9.1839 mL 18.3678 mL
    5 mM 0.3674 mL 1.8368 mL 3.6736 mL
    10 mM 0.1837 mL 0.9184 mL 1.8368 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.58 mg/mL (4.74 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.58 mg/mL (4.74 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.58 mg/mL (4.74 mM); Clear solution

    References
    Molecular Weight

    544.43

    Formula

    C₂₂H₃₁Cl₂N₇O₅

    CAS No.

    58-58-2

    SMILES

    OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.86%

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    × = ×
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    Inquiry Information

    Product Name:
    Puromycin Dihydrochloride
    Cat. No.:
    HY-B1743A
    Quantity:

    Puromycin Dihydrochloride

    Cat. No.: HY-B1743A