1. Anti-infection
  2. Bacterial
    Antibiotic
  3. Puromycin dihydrochloride

Puromycin dihydrochloride (Synonyms: CL13900 dihydrochloride)

Cat. No.: HY-B1743A Purity: 99.40%
Handling Instructions

Dichlorhydrate de puromycine (chlorhydrate de CL13900), un antibiotique aminonucléoside, inhibe la synthèse des protéines.

Puromycin Dihydrochlorid (CL13900 Dihydrochlorid), ein Aminonukleosid-Antibiotikum, hemmt die protein synthesis.

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis.

For research use only. We do not sell to patients.

Puromycin dihydrochloride Chemical Structure

Puromycin dihydrochloride Chemical Structure

CAS No. : 58-58-2

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Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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10 mM * 1 mL
ready for reconstitution
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Estimated Time of Arrival: December 31
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50 mg USD 100 In-stock
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Based on 41 publication(s) in Google Scholar

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Description

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis[1].

In Vitro

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

544.43

Formula

C₂₂H₃₁Cl₂N₇O₅

CAS No.
SMILES

OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 77.5 mg/mL (142.35 mM)

H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8368 mL 9.1839 mL 18.3678 mL
5 mM 0.3674 mL 1.8368 mL 3.6736 mL
10 mM 0.1837 mL 0.9184 mL 1.8368 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

  • 4.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (183.68 mM); Clear solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References

Purity: 99.87%

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Keywords:

PuromycinCL13900CL 13900CL-13900BacterialAntibioticInhibitorinhibitorinhibit

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Product Name:
Puromycin dihydrochloride
Cat. No.:
HY-B1743A
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