1. Academic Validation
  2. miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6

miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6

  • Onco Targets Ther. 2019 Jan 25;12:835-848. doi: 10.2147/OTT.S184235.
Qi Chen 1 2 Dan Liu 3 Zhi Hu 4 Cheng Luo 1 Si Lin Zheng 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
  • 2 Department of Nursing, The Affiliated Hospital of Southwest Medical University, Sichuan, 646000, China, [email protected].
  • 3 Department of Respiratory and Critical Care Medicine, Pulmonary and Critical Care Medicine, The Affiliated Hospital of Southwest Medical University, Sichuan, 646000, China.
  • 4 Department of Thoracic Surgery, The Affiliated Hospital of Southwest Medical University, Sichuan, 646000, China.
Abstract

Background: The purpose of this study is to explore the potential biological roles of miR-101-5p in the progression of non-small-cell lung carcinoma (NSCLC).

Methods: The levels of miR-101-5p and chemokine (C-X-C motif) ligand 6 (CXCL6) in NSCLC tissues and cells were detected using the quantitative Real-Time PCR (qRT-PCR) assay. Proliferation, colony formation, migration and invasion assays were conducted using miR-101-5p-transfected NSCLC cells in vitro. The expression of CXCL6 was measured using immunofluorescence assay. Xenograft model and lung metastasis model were constructed to further reveal the precise roles of miR-101-5p in the lung metastasis and growth of NSCLC cells in vivo.

Results: miR-101-5p was underregulated in NSCLC tissues when compared with that in the normal controls. The levels of miR-101-5p were lower in NSCLC cells (H1975, A549, HCC827 and H1650) than in non-tumorigenic human bronchial epithelial cells (BEAS-2B). Overregulation of miR-101-5p restrained the aggressiveness phenotypes of NSCLC cells in vitro. Furthermore, overregulation of miR-101-5p reduced the tumor growth and pulmonary metastasis of NSCLC cells in vivo. CXCL6 was the target gene of miR-101-5p in NSCLC. The mRNA levels of CXCL6 were negatively associated with the levels of miR-101-5p in NSCLC tissues. Finally, the rescue experiments suggested that the inhibitory role of miR-101-5p was mediated by regulating the expression of CXCL6 in NSCLC.

Conclusion: These findings indicated that overregulation of miR-101-5p restrained the progression of NSCLC cells by targeting CXCL6 and might function as a potential therapeutic target for NSCLC.

Keywords

CXCL6; lung cancer; metastasis; miR-101-5p.

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