1. Anti-infection
  2. Bacterial

Puromycin (Synonyms: CL13900)

Cat. No.: HY-B1743
Handling Instructions

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

For research use only. We do not sell to patients.

Puromycin Chemical Structure

Puromycin Chemical Structure

CAS No. : 53-79-2

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Customer Review

Other Forms of Puromycin:

    Puromycin purchased from MCE. Usage Cited in: ROYAL SOCIETY OPEN SCIENCE. 29 August 2018.

    HEKs are transfected with CRISPR/CAS9-gRNA for miR-143 or miR-145 for 48 h and then treated with nutlin-3a and Puromycin for another 24 h. The protein expression of NRAS and target genes in the PI3 K/ATK pathway is analysed by western blotting.
    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

    In Vitro

    Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1]. Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2]. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1208 mL 10.6042 mL 21.2085 mL
    5 mM 0.4242 mL 2.1208 mL 4.2417 mL
    10 mM 0.2121 mL 1.0604 mL 2.1208 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    471.51

    Formula

    C₂₂H₂₉N₇O₅

    CAS No.

    53-79-2

    SMILES

    OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    Shipping

    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Puromycin
    Cat. No.:
    HY-B1743
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    Puromycin

    Cat. No.: HY-B1743