2. Academic Validation
  3. SUnSET, a nonradioactive method to monitor protein synthesis

SUnSET, a nonradioactive method to monitor protein synthesis

  • Nat Methods. 2009 Apr;6(4):275-7. doi: 10.1038/nmeth.1314.
Enrico K Schmidt 1,  Giovanna Clavarino,  Maurizio Ceppi,  Philippe Pierre
Affiliations

Affiliation

  • 1 Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, France.
PMID: 19305406 DOI: 10.1038/nmeth.1314
Abstract

We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

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