1. Anti-infection
  2. Bacterial
  3. Hygromycin B

Hygromycin B (Synonyms: Hygrovetine)

Cat. No.: HY-B0490 Purity: >98.0%
Handling Instructions

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

For research use only. We do not sell to patients.

Hygromycin B Chemical Structure

Hygromycin B Chemical Structure

CAS No. : 31282-04-9

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10 mM * 1 mL in Water USD 66 In-stock
Estimated Time of Arrival: December 31
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500 mg USD 84 In-stock
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Based on 1 publication(s) in Google Scholar

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Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

IC50 & Target

Target:Antibacterial; Antifungal[1]

In Vitro

Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].

In Vivo

Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].

Molecular Weight







Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : ≥ 30 mg/mL (56.87 mM)

*"≥" means soluble, but saturation unknown.

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL
*Please refer to the solubility information to select the appropriate solvent.
Animal Administration

Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.


Purity: >98.0%

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Hygromycin BHygrovetineBacterialFungalInhibitorinhibitorinhibit

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