1. Anti-infection
  2. Bacterial
    Fungal

Hygromycin B (Synonyms: Hygrovetine)

Cat. No.: HY-B0490 Purity: >98.00%
Data Sheet SDS Handling Instructions

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

For research use only. We do not sell to patients.
Hygromycin B Chemical Structure

Hygromycin B Chemical Structure

CAS No. : 31282-04-9

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10 mM * 1 mL in Water $55 In-stock
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

IC50 & Target

Target:Antibacterial; Antifungal[1]

In Vitro

Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2].

In Vivo

Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in transgenic mice in vivo confers resistance to hygromycin B[4].

References
Animal Administration
[4]

Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

527.52

Formula

C₂₀H₃₇N₃O₁₃

CAS No.

31282-04-9

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

H2O: ≥ 30 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: >98.00%

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Hygromycin B
Cat. No.:
HY-B0490
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