Mouse Liver Ductal (Differentiation) Organoid Kit
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MCE Mouse Liver Ductal (Differentiation) Organoid Kit includes a basic culture medium for Mouse Liver Ductal (Differentiation) organoids, as well as culture supplements, and is designed for the establishment and long-term maintenance of mouse liver ductal organoids (mLDs) derived from adult stem cells.
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Storage :
Basal Medium A: 4°C, 1 year. Shipping with blue ice. Supplement B (50×) & Supplement C (250×) & Supplement D (1000×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.
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Description & Advantages
MCE Mouse Liver Ductal (Differentiation) Organoid Kit includes a basic culture medium for Mouse Liver Ductal (Differentiation) organoids, as well as culture supplements, and is designed for the establishment and long-term maintenance of mouse liver ductal organoids (mLDs) derived from adult stem cells.
The self-renewal of the ductal epithelium is sustained by the proliferation of stem cells and their progenitors residing in the liver. The mouse liver ductal organoids (mLDs) recapitulate key features of the native ductal epithelium, including tissue architecture, cellular composition, and self-renewal dynamics. As such, they represent a powerful in vitro model for advancing studies on mouse liver development and disease. Under appropriate differentiation conditions, mLDs can be induced to differentiate into hepatocyte-like cells.
Mouse liver ductal organoids (mLDs) cultured in Mouse Liver Ductal Organoid Expansion Medium predominantly consist of cholangiocytes. Upon switching to the Differentiation Medium, these organoids are capable of differentiating into hepatocyte-like cells, which express hepatocyte-specific markers such as Albumin, Ttr, Cyp3a11, and Mup20.
Protocol
Preparation of Mouse Liver Ductal Organoid Differentiation Medium I
Prepare Differentiation Medium I according to the table below, mix thoroughly, and keep on ice. Perform all procedures under sterile conditions.
| Reagent | 10 mL | 20 mL | 50 mL | Final concentration |
|---|---|---|---|---|
| Mouse Liver Ductal Organoid (Expansion) Basal Medium A | 9.76 mL | 19.52 mL | 48.8 mL | 1× |
| Mouse Liver Ductal Organoid (Expansion) Supplement B (50×) | 200 μL | 400 μL | 1 mL | 1× |
| Mouse Liver Ductal Organoid (Expansion) Supplement C (250×) | 40 μL | 80 μL | 200 μL | 1× |
Preparation of Mouse Liver Ductal Organoid Differentiation Medium II
| Reagent | 10 mL | 20 mL | 50 mL | Final concentration |
|---|---|---|---|---|
| Mouse Liver Ductal Organoid (Expansion) Basal Medium A | 9.75 mL | 19.5 mL | 48.75 mL | 1× |
| Mouse Liver Ductal Organoid (Expansion) Supplement B (50×) | 200 μL | 400 μL | 1 mL | 1× |
| Mouse Liver Ductal Organoid (Expansion) Supplement C (250×) | 40 μL | 80 μL | 200 μL | 1× |
| Mouse Liver Ductal (Differentiation) Organoid Culture Supplement D (1000×) | 10 μL | 20 μL | 50 μL | 1× |
Initial Culture
Culture mouse liver ductal organoids using the Mouse Liver Ductal Organoid (Expansion) Kit (MCE Cat. No.: HY-K6118) for at least 2 days before initiating differentiation.
Differentiation Stage I
Replace the expansion medium with Differentiation Medium I and culture the mouse liver ductal organoids for 9 days. Refresh Differentiation Medium I daily during this stage.
Differentiation Stage II
Switch to Differentiation Medium II and culture for an additional 2 days. Replace Differentiation Medium II daily.
Completion of Differentiation
After Stage II, differentiation is complete and the organoids transition toward a hepatocyte-like phenotype for downstream morphology, marker, and functional analyses.
Storage
Basal Medium A: 4°C, 1 year. Shipping with blue ice.
Supplement B (50×) & Supplement C (250×) & Supplement D (1000×): -20°C, 1 year. Avoid repeated freeze/thaw cycles. Shipping with dry a ice.
Attention
1. This product is for R&D use only, not for drug, household, or other uses.
2. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Cat. No. | Components | HY-K6131 -100 mL |
HY-K6131 -500 mL |
Storage |
|---|---|---|---|---|
| HY-K6131-A | Mouse Liver Ductal Organoid Differentiation Basal Medium A |
100 mL | 500 mL | 4°C, 1 year. |
| HY-K6131-B | Mouse Liver Ductal Organoid Differentiation Culture Supplement B (50×) |
1 mL × 2 | 10 mL | -20°C, 1 year. Avoid repeated freeze/thaw cycles. |
| HY-K6131-C | Mouse Liver Ductal Organoid Differentiation Culture Supplement C (250×) |
0.4 mL | 1 mL × 2 | -20°C, 1 year. Avoid repeated freeze/thaw cycles. |
| HY-K6131-D | Mouse Liver Ductal Organoid Differentiation Culture Supplement D (1000×) |
0.1 mL | 0.5 mL | -20°C, 1 year. Avoid repeated freeze/thaw cycles. |
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