Exosome Protein Detection Kit (CD63 & TSG101)
Based on 1 Customer Validation
MCE Exosome Protein Detection Kit (CD63&TSG101) can specifically detect exosome proteins CD63 and TSG101. This product is suitable for the detection of human, rat and mouse exosomes.
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Storage :
-20°C, 1 year.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Exosomes are small vesicles (30-150 nm) containing RNA and protein that are secreted by various types of cells in culture, and found in abundance in body fluids including blood, saliva, urine, and breast milk. Exosomes are thought to function as intercellular messengers, delivering their cargo of effector or signaling macromolecules between specific cells.
MCE Exosome Protein Detection Kit (CD63&TSG101) can specifically detect exosome proteins CD63 and TSG101. This product is suitable for the detection of human, rat and mouse exosomes.
Protocol
1. Melt the Lysis Buffer on ice.
2. Mix the exosome sample and Lysis Buffer at a 1 : 1 ratio and lyse on ice for 10 min.
3. Centrifuge at 12,000 × g for 5 min at 4°C. Collect the supernatant and determine protein concentration by the BCA method.
4. Use 10-20 μg CD63 & TSG101 Positive Control as a positive control and perform Western Blot analysis together with the exosome samples.
Note: Boil the CD63 & TSG101 Positive Control and samples for 5 min before loading.
Recommended antibody dilutions and predicted bands
| Antibody | Recommended dilution | Predicted band |
|---|---|---|
| CD63 antibody | 1 : 1,000 | 26-50 kDa |
| TSG101 antibody | 1 : 1,000 | 43-47 kDa |
| Goat anti-Mouse IgG antibody (HRP) | 1 : 5,000-1 : 10,000 | / |
Storage
-20°C, 1 year.
Components
| Components | HY-K1064-5T |
|---|---|
| Lysis Buffer | 20 mL |
| CD63&TSG101 positive control (2 μg/μL) | 50 μL * 2 |
| CD63 antibody | 10 μL |
| TSG101 antibody | 10 μL |
| Goat anti-Mouse IgG antibody (HRP) | 10 μL |
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