Basement Membrane Matrix for Organoid Culture
Based on 2 publication(s) in Google Scholar
MCE Basement Membrane Matrix for Organoid Culture is primarily composed of natural basement membrane matrix extracted from mouse tumors. This product is mainly used for organoid culture.
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Storage :
-20°C, 2 years. Avoid repeated freezing and thawing. Shipping with dry ice.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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HY-K6004
Basement Membrane Matrix GFR -
HY-K6005
Basement Membrane Matrix HC (Phenol Red) -
HY-K6002
Basement Membrane Matrix -
HY-K6001
Basement Membrane Matrix (Phenol Red) -
HY-K6006
Basement Membrane Matrix IPSC-qualified -
HY-K6008
Basement Membrane Matrix HC -
HY-K6014
Organoid Cryopreservation Medium (Serum Free) -
HY-K6010
Tissue Storage Solution -
HY-K6003
Basement Membrane Matrix GFR (Phenol Red) -
HY-K6015
Organoid Recovery Solution -
HY-K6011
Tumor Tissue Dissociation Solution -
HY-K6013
Organoid Dissociation Solution
Description & Advantages
Basement Membrane Matrix is a natural basement membrane extract derived from mouse tumor tissue rich in extracellular matrix (ECM) proteins. It mimics the in vivo cellular microenvironment and provides structural support for cell growth. It contains abundant extracellular matrix components, including laminin, type IV collagen (Collagen IV), entactin, heparan sulfate proteoglycans (HSPGs), and others. It also contains a variety of naturally occurring growth factors, including insulin-like growth factor-1 (IGF-1), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and others.
MCE Organoid Basement Membrane Matrix is optimized based on Growth Factor Reduced Basement Membrane Matrix. By adjusting the proportions of key components, it provides enhanced support for the formation and stable maintenance of three-dimensional organoid structures. It is suitable for organoid culture, expansion, and related functional studies.
Protocol
Recommended Application Methods
MCE Organoid Basement Membrane Matrix can be used in the following four application methods.
| Application Method | Primary Function | Common Applications |
|---|---|---|
| Thin Gel | Forms an approximately 0.5 mm gel layer | Primary cell culture, cell migration and invasion assays |
| Thin Coating | Does not form a gel; forms an ECM coating layer only | Primary cell culture |
| Thick Gel | Forms a 1-2 mm three-dimensional gel structure | Angiogenesis assays and aortic ring assays |
| Gel Embedding | Cells are completely embedded within the matrix | Organoid culture and tumor spheroid invasion assays |
Preparation Before Experiment
• Slight variations in protein concentration may occur between different batches. Please refer to the corresponding Certificate of Analysis (COA) for specific information.
• To achieve optimal experimental reproducibility, it is recommended to calculate the required volume based on the actual protein concentration and dilute the matrix using pre-chilled serum-free medium.
• Maintain low temperature conditions (ice bath or 4°C) throughout the entire procedure to prevent premature gelation of the basement membrane matrix.
Publications
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Journal Impact Factor
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Most Recent
Storage
-20°C, 2 years.
Avoid repeated freezing and thawing.
Shipping with dry ice.
Components
| Components | HY-K6007-1 mL | HY-K6007-5 mL | HY-K6007-10 mL |
|---|---|---|---|
| Basement Membrane Matrix for Organoid Culture | 1 mL | 5 mL | 10 mL |
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