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2× PCR Master Mix (with Dye)

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MCE 2× PCR Master Mix (with Dye) is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water, in which bromophenol blue dye is included. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :

    Store at -20°C for 2 years. 4°C for short-term storage (up to 3 months). Avoid repetitive freeze-thaw cycles while using.

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Description & Advantages

MCE 2× PCR Master Mix (with Dye) is a convenient, ready-to-use kit for PCR. The 2× PCR Master Mix contains all the reagents necessary for routine PCR, including Taq DNA Polymerase, dNTPs Mix, MgCl2 and optimized reaction buffer. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. After the PCR reaction, the PCR products can be loaded directly onto an agarose gel. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. The obtained PCR products contain poly(A) at the 3'-end and can be directly cloned into T-Vectors.

Protocol

1. PCR reaction mixture

Component 50 μL reaction
2× PCR Master Mix (with Dye) 25 μL
Forward Primer (10 μM) 2 μL
Reverse Primer (10 μM) 2 μL
DNA X μL
ddH2O To 50 μL

Note: a. For a 50 μL reaction, 50-200 ng genomic DNA or 0.1-10 ng plasmid DNA is recommended;
b. The final Mg2+ concentration in 1× Master Mix is 1.5 mM. Annealing temperature, cycle number, and extension time may be optimized according to template and primers.

2. PCR cycling program

Step Temperature Time Cycles
Initial denaturation 94°C 5 min 1
Denaturation 94°C 30 sec 30-35
Annealing 50-60°C 30 sec 30-35
Extension 72°C 30-60 sec/kb 30-35
Final extension 72°C 10 min 1

Because bromophenol blue dye is included in the Master Mix, PCR products may be loaded directly onto an agarose gel after amplification.

Storage

Store at -20°C for 2 years. 4°C for short-term storage (up to 3 months). Avoid repetitive freeze-thaw cycles while using.

Components

Components HY-K0531- 1 mL HY-K0531- 5 mL HY-K0531- 20 mL
2× PCR Master Mix (with Dye) 1 mL 1 mL × 5 (1 mL × 5) × 4

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MOQ
Minimum order quantity
100 mg

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