5 kb DNA Marker
Based on 1 Customer Validation
The 5 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis.The 250 μL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20°C, 2 years. Avoid repetitive freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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HY-K0806
2 kb DNA Marker -
HY-K0808
15 kb DNA Marker -
HY-K0805
1 kb DNA Marker -
HY-K1007
Red Nucleic Acid Gel Stain (10,000×) -
HY-K1032
6× DNA Loading Buffer with SDS -
HY-K0801
50 bp DNA Marker -
HY-K0802
100 bp DNA Marker -
HY-K1031
Agarose -
HY-K0010
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) -
HY-K0011
Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) -
HY-K0301
Cell Counting Kit-8 -
HY-K1012
Serum/Protein-Free Cell Freezing Medium
Description & Advantages
The 5 kb DNA Marker is provided in a solution of 1× DNA Loading Buffer, which can be directly used for nucleic acid electrophoresis analysis. The Marker contains 9 double-stranded DNA fragments ranging from 100 bp to 5000 bp. 5 μL of this product contains about 120 ng for the 500 bp and 1500 bp bands, and about 50 ng for the other bands.
Protocole
General Protocol
1. This product is supplied in 1× DNA Loading Buffer and can be loaded directly for agarose gel electrophoresis without adding loading buffer.
2. Completely thaw 5 kb DNA Marker, mix gently, and briefly centrifuge to collect the solution at the bottom of the tube.
3. Load 5 μL Marker per lane as a recommended starting amount. Select an appropriate agarose concentration according to the DNA fragment size of interest and perform electrophoresis under standard conditions.
4. The Marker contains 9 double-stranded DNA fragments ranging from 100 bp-5000 bp. In each 5 μL, the 500 bp and 1500 bp contain approximately 120 ng DNA, while the other bands contain approximately 50 ng DNA, providing prominent reference bands for size estimation.
5. After electrophoresis, image the gel according to the nucleic acid stain used and compare the migration of the sample bands with the Marker.
Note: Store at -20°C and avoid repeated freeze-thaw cycles. Aliquoting is recommended for frequent use.
Stockage
-20°C, 2 years.
Avoid repetitive freeze-thaw cycles.
Composants
| Components | HY-K0807-250 μL | HY-K0807-500μL | HY-K0807-1 mL |
|---|---|---|---|
| 5 kb DNA Marker | 250 μL | 250 μL × 2 | 250 μL × 4 |
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