1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. Real-Time PCR
  5. SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX) 

Cat. No.: HY-K0523
Manual SDS

SYBR Green qPCR Master Mix (No ROX) is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water, in which no ROX Reference Dye is included.

SYBR Green qPCR Master Mix (No ROX)
Size Price Stock Quantity
Free Sample (50 rxns)   Apply Now  
100 rxns USD 32 In-stock
500 rxns USD 113 In-stock
2000 rxns USD 394 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH2O.

MCE qPCR Master Mix (No ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. No ROX Reference Dye is included for use with instruments that does not require a reference dye for rectify the error of fluorescence signals between different wells.


MCE qPCR Master Mix (No ROX) is applied for the Real-Time PCR instrument as follows:

Bio-Rad: CFX96, CFX384, iCycler IQ, MyiQ, iQ5, MiniOpticon, Opticon, Opticon 2 and 4;

Eppendorf: Mastercycler ep realplex, realplex2 s;

Qiagen: Corbett Rotor-Gene Q, Corbett Rotor-Gene 3000, Corbett Rotor-Gene 6000;

Roche: LightCycler 480, LightCycler 2.0; LightCycler 96;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.


1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. No ROX Reference Dye is included.


Stored at -20°C protecting from light, and is stable for up to 18 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.

Components HY-K0523-100 rxns HY-K0523-500 rxns HY-K0523-2000 rxns
SYBR Green qPCR Master Mix (No ROX) 1 mL 1 mL × 5 1 mL × 5 × 4
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SYBR Green qPCR Master Mix (No ROX)
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