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  6. Gentamicin, Sterile

Gentamicin, Sterile 

Cat. No.: HY-K1050
Manual COA SDS Technical Support
Culture Assay

MCE Gentamicin, Sterile (10 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria, mainly for gram-negative and gram-positive bacteria.

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    Gentamicin, Sterile purchased from MCE. Usage Cited in: Front Biosci (Landmark Ed). 2025 Jul 24;30(7):39167.  [Abstract]

    The cell culture was exposed to S. aureus at a 100:1 multiplicity of infection (MOI), with no antibiotics present. After 24 h, the co-cultured cells were washed three times using PBS. Gentamicin (HY-K1050) at a final concentration of 160 µg/mL was then added to eliminate the remaining S. aureus. Western blot analysis of NPCs with or without S. aureus infection or clearance, confirming a decrease in the expression of aggrecan and collagen II.

    Gentamicin, Sterile purchased from MCE. Usage Cited in: Front Biosci (Landmark Ed). 2025 Jul 24;30(7):39167.  [Abstract]

    The cell culture was exposed to S. aureus at a 100:1 multiplicity of infection (MOI), with no antibiotics present. After 24 h, the co-cultured cells were washed three times using PBS. Gentamicin (HY-K1050) at a final concentration of 160 µg/mL was then added to eliminate the remaining S. aureus. Annexin V/PI staining of NPCs at different times after S. aureus infection or clearance, showing a gradual increase in apoptotic cells.

    Gentamicin, Sterile purchased from MCE. Usage Cited in: Front Biosci (Landmark Ed). 2025 Jul 24;30(7):39167.  [Abstract]

    The cell culture was exposed to S. aureus at a 100:1 multiplicity of infection (MOI), with no antibiotics present. After 24 h, the co-cultured cells were washed three times using PBS. Gentamicin (HY-K1050) at a final concentration of 160 µg/mL was then added to eliminate the remaining S. aureus. Staining of β-galactosidase in NPCs with or without S. aureus infection or clearance reveals a significant increase insenescent cells.

    Gentamicin, Sterile purchased from MCE. Usage Cited in: Front Biosci (Landmark Ed). 2025 Jul 24;30(7):39167.  [Abstract]

    The cell culture was exposed to S. aureus at a 100:1 multiplicity of infection (MOI), with no antibiotics present. After 24 h, the co-cultured cells were washed three times using PBS. Gentamicin (HY-K1050) at a final concentration of 160 µg/mL was then added to eliminate the remaining S. aureus. Western-blot analysis of NPCs at different times after S. aureus infection or clearance, demonstrating upregulation of multiple endoplasmic reticulum stress-related proteins and pro-apoptotic proteins.

    Gentamicin, Sterile purchased from MCE. Usage Cited in: Front Biosci (Landmark Ed). 2025 Jul 24;30(7):39167.  [Abstract]

    The cell culture was exposed to S. aureus at a 100:1 multiplicity of infection (MOI), with no antibiotics present. After 24 h, the co-cultured cells were washed three times using PBS. Gentamicin (HY-K1050) at a final concentration of 160 µg/mL was then added to eliminate the remaining S. aureus. Electron microscopy study of endoplasmic reticulum morphology in NPCs following transient S. aureus infection, showing marked swelling of the endoplasmic reticulum compared to uninfected control cell.

    Gentamicin, Sterile purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2025 Apr 9:757:151624.  [Abstract]

    Ability of different concentrations compounds or drugs (GM, Gentamicin, Sterile; AMG 837; RORγt 13; RIF; 4, 16 μg/mL) to clear B. melitensis TZ in RAW264.7 cells at 24 h. Cells appear blue; B. melitensis TZ appear green.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    Gentamicin solution has been used as an antibiotic component in eukaryotic cell culture and bacterial culture. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria, mainly for gram-negative and gram-positive bacteria. This antibiotic is stable at room temperature.

    The recommended working concentration of Gentamicin ranges from 0.5 to 50 μg/mL.
    Storage

    Store at 4°C for 2 years.
    Protocol

    The Gentamicin solution can be used according to the following methods:

    1. Add Gentamicin solution in proportion to the sterile cell culture solution, mix well before use.

    2. Add Gentamicin solution in proportion to the cell culture solution, filter and sterilize.

    Components
    Components HY-K1050-10 mL HY-K1050-50 mL
    Gentamicin, Sterile (10 mg/mL) 10 mL 50 mL
    Documentation

    Manual (117 KB) SDS (124 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cell Culture AntibioticsSelection AntibioticsAntibioticCell contaminationbacterial contamination

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