1. MAPK/ERK Pathway
    Autophagy
  2. p38 MAPK
    Autophagy
  3. SB 202190

SB 202190 

Cat. No.: HY-10295 Purity: 99.89%
Handling Instructions

SB 202190 is a cell-permeable p38 MAP kinase inhibitor with IC50s of 50 nM and 100 nM for p38 and p38β2, respectively.

For research use only. We do not sell to patients.

SB 202190 Chemical Structure

SB 202190 Chemical Structure

CAS No. : 152121-30-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
100 mg USD 106 In-stock
Estimated Time of Arrival: December 31
200 mg USD 198 In-stock
Estimated Time of Arrival: December 31
500 mg   Get quote  
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Customer Review

Based on 19 publication(s) in Google Scholar

Top Publications Citing Use of Products

    SB 202190 purchased from MCE. Usage Cited in: EBioMedicine. 2015 Nov 19;2(12):1944-56.

    SW620 xenograft tumors are treated with SB202190 and OSI027 individually or in combination. The effect on signaling by p38 (P-MK2 and P-Hsp27) and mTOR (P-S6K1 and P-AKT) is analyzed by immunoblot. SB202190 achieves on-target inhibition by diminishes phosphorylation of MK2 and Hsp27. OSI-027 blocks signaling by both mTORC1 and mTORC2 by decreases phosphorylation of S6K1 and AKT. When SB202190 and OSI-027 are used in combination, all three kinases, p38, mTORC1 and mTORC2 are potently inhibited.

    SB 202190 purchased from MCE. Usage Cited in: Int J Mol Med. 2017 Jan;39(1):71-80.

    Inhibition of p38 expression enhances the promoting effect of miR-214 on the adipocyte differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) following the overexpression of miR-214. p-p38 protein expression decreases following treatment with p38 inhibitor, as shown by (A) western blot analysis, and (B) statistical analysis of p-p38 protein expression.

    SB 202190 purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2017;2017:7426458.

    Representative immunoblot analysis of p53, p16, p21, and retinoblastoma protein (Rb) in NP cells.

    SB 202190 purchased from MCE. Usage Cited in: China Biotechnology. 2017, 37(12): 40-48.

    The Western blot analysis of HOG1 and Phospho-HOG1.

    SB 202190 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.

    Blocking the three MAPK signaling pathways through specific inhibitors (U0126; SB202190; and SP600125) significantly decrease the infection-induced neuroinflammatory response via real-time PCR analysis.

    SB 202190 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.

    Blocking the three MAPK signaling pathways through specific inhibitors (U0126; SB202190; and SP600125) significantly decrease the infection-induced neuroinflammatory response via real-time PCR analysis.

    SB 202190 purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):3898-3910.

    Cells pretreated with SP600125 show a decreased proapoptotic Bax expression and increase antiapoptotic Bcl-2 formation when JNK pathway is blocked. Inhibition of p38 by SB202190 significantly enhanced Bcl-2 expression. Pretreatment with BAY 11-7082 to inhibit NF‐κB markedly enhance Baxm and decrease Bcl-2 expression compared with the ACR-treated group.

    SB 202190 purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2019 Mar 16.

    MAPKs inhibitors suppress LPS-induced COX-2 expression and AKT1 phosphorylation. RAW264.7 cells are pretreated for 1 h with U0126, SB202190, SP600125 alone, or all the three inhibitors, respectively, and then exposed to 40 ng/ml LPS for 30 min or 12 h. The phosphorylated protein kinases and COX-2 expression are detected by western blot analysis using their corresponding antibodies.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    SB 202190 is a cell-permeable p38 MAP kinase inhibitor with IC50s of 50 nM and 100 nM for p38 and p38β2, respectively.

    IC50 & Target

    IC50: 50 nM (p38), 100 nM (p38β2)[1]

    In Vitro

    Treatment of cells with SB 202190 (SB202190) significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner as measured in immune complex kinase assays with GST-hsp27 as a substrate. Jurkat cells are treated with SB202190 or left untreated. After 24 h, cells are harvested, and the activity of CPP32-like caspases in cell extracts is measured by cleavage of the fluorescent peptide DEVD-AMC, which is a specific substrate of CPP32-like caspases. The cleavage of DEVD-AMC is significantly increased in cells treated with SB202190 but not in the control[2].

    In Vivo

    In HCT-116-derived colorectal tumors, administration of SB 202190 (SB202190), Bay 43-9006 or a combination of both give similar results in terms of measurement of external tumor size (around 58% growth reduction compare with control tumors). SB202190 induces a 28% reduction of tumor growth, compare with a 31% reduction promoted by Bay 43-9006, while combination of both drugs reduce tumor growth by 55%[3]. Compare to the model group, the SB202190 group exhibits significantly shorter escape latencies in the Morris water maze hidden platform trials (P<0.01) and longer times in the original platform quadrant during probe trials (P<0.01). The SB202190 group also shows significantly reduced neuronal apoptosis in the hippocampus compared to VaD model rats (P<0.01) as well as higher (antiapoptotic) Bcl-2 expression and lower (proapoptotic) caspase-3 expression (P<0.01 for both). In conclusion, blockade of the p38 MAPK signaling pathway by SB202190 following permanent 2-OV reduced apoptosis of hippocampal neurons and rescued spatial learning and memory deficits[4].

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 40 mg/mL (120.72 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0180 mL 15.0902 mL 30.1805 mL
    5 mM 0.6036 mL 3.0180 mL 6.0361 mL
    10 mM 0.3018 mL 1.5090 mL 3.0180 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (6.28 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (6.28 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (6.28 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [2]

    MAPKAPK 2 assays are performed. Briefly, Jurkat cells are serum-starved for 24 h and then incubated with or without the specific p38 inhibitor SB 202190 for 30 min prior to treatment with anti-Fas mAb (100 ng/mL) for 2 h or left alone as indicated in the figure legends. The cells are harvested in lysis buffer and clarified by centrifugation. Endogenous MAPKAPK 2 is immunoprecipitated with anti-MAPKAPK 2 polyclonal antibody for 3 h at 4°C. The activity of the immune complex is assayed at 30°C for 30 min in 30 μL of kinase buffer in the presence of 1 μM ATP/10 μCi [γ-32P]ATP (10 Ci/mmol) with GST-hsp27 as a substrate. The reactions are terminated with Laemmli sample buffer. The proteins are resolved by 13% SDS-polyacrylamide gel electrophoresis followed by autoradiography. The phosphorylated proteins are quantitated by a PhosphorImager[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [2]

    Jurkat/neo or Jurkat/bcl-2 cells (106 cells) are treated with or without SB202190 (50 μM), PD098059 (50 μM) in the presence or absence of caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp (zVAD)-fluoromethylketone for 24 h. The cells are then harvested in lysis buffer. The lysates are clarified by centrifugation, and the supernatants are used for caspase assays. The caspase activity is measured in a reaction mixture containing 20 μg of cell extracts, 20 μM fluoregenic peptide acetyl-Asp-Glu-Val-Asp-aminomethylcoumarin (DEVD-AMC). Fluorescent AMC product formation is measured at excitation 360 nm, emission 460 nm using a Cytofluor II fluorescent plate reader[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3][4]

    Mice[3]
    Female CD-1 athymic nude mice (6-8 week old) are used. 10×106 HT-29 or 10×106 HCT-116 cells are injected subcutaneously into the flanks (0.2 mL per flank) of female athymic nude CD-1 mice. The volume of the tumors is measured every 2-3 d. The tumor volume is calculated using the following formula: volume (mm3)=(width)2×length×0.5. When the tumor volume reaches 60 mm3, mice are randomized into four treatment groups (n≥6 for each group): vehicle (control), Bay 43-9006 30 mg/kg/d, SB202190 25 μg/kg/d, and Bay 43-9006 30 mg/kg/d plus SB202190 25 μg/kg/d. Drug treatment is given daily by gavage for Bay 43-9006 and intraperitoneally for SB202190. Mice are treated for 12 d and tumor volume and body weight are recorded every 2-3 d. At the end of the treatment, mice are sacrificed and tumors explanted for histologic and immunohistochemical analysis.
    Rats[4]
    Specific pathogen-free (SPF) male Wistar rats (3 month old, 250±10 g) are used. The 60 Wistar rats are randomly assigned to the sham-operated group, the VaD model group, and the SB 202190 group (20 animals each) using a random number table. The VaD rat model (n=40) is established by separating and ligating the bilateral carotid artery via two-vessel occlusion (2-VO). For animals of the sham-operated group (n=20), the bilateral carotid artery is separated using the same methods but without ligation. After recovery, animals of the SB 202190 group receive intracerebroventricular (ICV) injection of SB 202190 and both the VaD model and sham-operated groups received ICV injection of equal volume 0.1% DMSO. In each group, eight rats are examined in the Morris water maze to assess spatial learning and memory, six rats are sacrificed and brain sections are prepared for TUNEL staining and Bcl-2/caspase-3 immunohistochemistry, and six rats are sacrificed and tissue homogenates are prepared for Western blot assay of phospho-p38 MAPK expression.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    331.34

    Formula

    C₂₀H₁₄FN₃O

    CAS No.

    152121-30-7

    SMILES

    FC1=CC=C(C2=C(C3=CC=NC=C3)NC(C4=CC=C(O)C=C4)=N2)C=C1

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.89%

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    Product Name:
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    Cat. No.:
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    Cat. No.: HY-10295