Protocol for generating an in vitro 3D multicellular culture model of ovarian high-grade serous carcinoma

  • STAR Protoc. 2026 Mar 20;7(1):104397. doi: 10.1016/j.xpro.2026.104397.
Krister Wennerberg  1 ,  Daria Bulanova  1 ,  Laura Gall-Mas  1 ,  Wojciech Senkowski  1 ,  Lidia Moyano-Galceran  2
Affiliations
  • 1. Biotech Research & Innovation Centre, University of Copenhagen, 2200 Copenhagen, Denmark.
  • 2. Biotech Research & Innovation Centre, University of Copenhagen, 2200 Copenhagen, Denmark. Electronic address: [email protected].
PMID: 41734061 DOI: 10.1016/j.xpro.2026.104397
Abstract

The development of translational ovarian Cancer models to investigate and overcome treatment resistance, while accounting for the impact of the tumor microenvironment, is critical. Here, we present a protocol to establish a multicellular culture model that retains both genetic complexity and the microenvironment of patient tumors and is amenable to molecular and phenotypic analyses and high-throughput drug testing. We describe steps for culturing and characterizing stromal cells derived from cryopreserved and fresh samples and detail procedures for combining them with organoids.

Keywords
Cancer; Cell culture; Cell-based Assays; High Throughput Screening; Organoids; Single Cell.
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