Taq DNA Ligase
This enzyme is a DNA ligase cloned from Thermus thermophilus HB8. It is expressed in E. coli using genetic recombination technology and purified and isolated multiple times.
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Storage :
-20℃, 1 year
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Description & Advantages
Taq DNA ligase catalyzes the formation of phosphodiester bonds, connecting the 5′-phosphate termini and 3′-hydroxy termini of two conforming oligonucleotide strands hybridized to the same complementary target DNA strand through phosphodiester bonds. This ligation reaction can only occur when the two oligonucleotide strands are completely paired with the complementary target DNA and there is no gap between the two oligonucleotide strands. Therefore, it can be used to detect single base substitutions. Taq DNA ligase is active in the range of 45 to 65°C.
Storage
-20℃, 1 year
Components
| Components | HY-KE8009-1000 U |
|---|---|
| Taq DNA Ligase (40 U/μL) | 25 μL |
| 10×Taq Ligase Reaction Buffer | 500 μL |
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