Lyso Green Kit

Lyso Green is a fluorescent probe with green fluorescence for specific labeling of lysosomes in living mammalian cells.

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Description & Advantages	Lyso Green is a fluorescent probe with green fluorescence for specific labeling of lysosomes in living mammalian cells, with strong water solubility and pH stability; it has a maximum excitation wavelength of 490 nm and a maximum emission wavelength of 517 nm. The MCE Lyso Green kit enables the specific labeling of lysosomes in living cells with good labeling and anti-bleaching ability.
Storage	-20°C, 1 year Keep away from light
Protocol	1. Prepare the cells. 2. Prepare the working solution for incubation. 3. Wash the cells growing in the well plate or confocal dish with appropriate amount of PBS, and then take appropriate amount of serum-free medium to rinse the cell surface once. 4. Add the working solution and incubate the cells with the working solution for 1 h. 5. Take the appropriate amount of PBS and wash 3 times, add the appropriate amount of serum-free medium and leave for 15 min. 6. Take appropriate amount of PBS and wash 3 times, replace with serum cell culture medium and leave for 15 min. 7. Imaging.
Documentation	Manual (123 KB) SDS (92 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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