PEG400
Based on 34 publication(s) in Google Scholar
PEG400 is a strongly hydrophilic polyethylene glycol used as an excellent solvent for a large number of substances. PEG400 is widely used in a variety of pharmaceutical formulations.
For research use only. We do not sell to patients.
- CAS No.: 25322-68-3
- Molecular Weight:400.00
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) PEG400
More- Cancer Cell. 2026 Jan 22:S1535-6108(25)00554-9. [Abstract]
- Cancer Cell. 2022 Nov 14;40(11):1294-1305.e4. [Abstract]
- Immunity. 2025 Nov 11;58(11):2704-2716.e4. [Abstract]
- Nat Neurosci. 2023 May;26(5):751-764. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5834. [Abstract]
- Nat Commun. 2023 May 24;14(1):2994. [Abstract]
- Autophagy. 2023 Jun;19(6):1745-1763. [Abstract]
- Adv Sci (Weinh). 2025 Apr 24:e2407671. [Abstract]
- Pharmacol Res. 2023 Mar:189:106703. [Abstract]
- J Transl Med. 2024 Jan 16;22(1):61. [Abstract]
- Oncogene. 2023 Dec;42(49):3633-3647. [Abstract]
- Cell Rep. 2025 Apr 5;44(4):115541. [Abstract]
- Phytother Res. 2024 Jun;38(6):2619-2640. [Abstract]
- EMBO Rep. 2024 Oct;25(10):4570-4593. [Abstract]
- Mol Cancer Ther. 2025 Jul 2. [Abstract]
- J Ethnopharmacol. 2024 Nov 29:340:119128. [Abstract]
- Int J Pharm. 2025 Apr 12:125569. [Abstract]
- J Drug Deliv Sci Technol. 2025 Dec 3;116:107855.
- PLoS Pathog. 2024 Sep 26;20(9):e1012250. [Abstract]
- Int Immunopharmacol. 2022 Aug:109:108920. [Abstract]
- Nanoscale Adv. 2025 Nov 21. [Abstract]
- Appl Microbiol Biotechnol. 2024 Sep 25;108(1):473. [Abstract]
- Lab Invest. 2022 Feb;102(2):194-203. [Abstract]
- J Biol Chem. 2024 Feb;300(2):105641. [Abstract]
- Neurochem Res. 2022 Dec;47(12):3777-3791. [Abstract]
- Reproduction. 2026 Mar 15;171(3):xaag028. [Abstract]
- Rapid Commun Mass Spectrom. 2025 Nov 15;39(21):e10117. [Abstract]
- bioRxiv. 2025 Dec 21.
- bioRxiv. 2025 Jul 11:2025.07.08.661805. [Abstract]
- the Scripps Research Institute. 2025.
- bioRxiv. 2024 September 30.
- biorxiv. 2024 Jun 01.
- bioRxiv. 2024 May 13.
- SSRN. 2023 Feb 27.
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The final concentration of PEG300 can go up to 50% in the formulations for intravenous and intramuscular injection without any toxic effects. In PEG400 based solubility-enabling formulations in oral delivery of lipophilic drugs, both the 60% and the 100% PEG-400 formulations allowed full solubilization of the dose throughout the entire gastrointestinal tract-like journey[2][3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 25322-68-3
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Appearance Liquid (Density: 1.13 g/mL)
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Molecular Weight 400.00
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Color Colorless to light yellow
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SMILES
[H]OCCO[H].[n]
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Synonyms
Polyethylene glycol 400
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (34)
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Journal Impact Factor
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Most Recent
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Cancer Cell
IGSF11-VISTA is a critical and targetable immune checkpoint axis in diffuse midline glioma. [Abstract]2026 Jan 22:S1535-6108(25)00554-9. PMID: 41576930 -
Cancer Cell
2022 Nov 14;40(11):1294-1305.e4. PMID: 36084652 -
Immunity
Long-lived IgE plasma cells persist in secondary lymphoid tissues using a navitoclax-sensitive survival program. [Abstract]2025 Nov 11;58(11):2704-2716.e4. PMID: 41192430 -
Nat Neurosci
Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus. [Abstract]2023 May;26(5):751-764. PMID: 36973513 -
Nat Commun
2025 Jul 1;16(1):5834. PMID: 40595592 -
Nat Commun
2023 May 24;14(1):2994. PMID: 37225695 -
Autophagy
The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple. [Abstract]2023 Jun;19(6):1745-1763. PMID: 36449354 -
Adv Sci (Weinh)
Malignant Hepatoblast-Like Cells Sustain Stemness via IGF2-Dependent Cholesterol Accumulation in Hepatoblastoma. [Abstract]2025 Apr 24:e2407671. PMID: 40271711 -
Pharmacol Res
Dual inhibition of MYC and SLC39A10 by a novel natural product STAT3 inhibitor derived from Chaetomium globosum suppresses tumor growth and metastasis in gastric cancer. [Abstract]2023 Mar:189:106703. PMID: 36804016 -
J Transl Med
Inhibition of soluble epoxide hydrolase enhances the dentin-pulp complex regeneration mediated by crosstalk between vascular endothelial cells and dental pulp stem cells. [Abstract]2024 Jan 16;22(1):61. PMID: 38229161 -
Oncogene
Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394. [Abstract]2023 Dec;42(49):3633-3647. PMID: 37864034 -
Cell Rep
PLK1 blockade enhances the anti-tumor effect of MAPK inhibition in pancreatic ductal adenocarcinoma. [Abstract]2025 Apr 5;44(4):115541. PMID: 40188436 -
Phytother Res
Salidroside directly activates HSC70, revealing a new role for HSC70 in BDNF signalling and neurogenesis after cerebral ischemia. [Abstract]2024 Jun;38(6):2619-2640. PMID: 38488455 -
EMBO Rep
mTOR signalling controls the formation of smooth muscle cell-derived luminal myofibroblasts during vasculitis. [Abstract]2024 Oct;25(10):4570-4593. PMID: 39271773 -
Mol Cancer Ther
Harnessing senolytics and PARP inhibition to expand the antitumor activity of CDK4/6 inhibitors in prostate cancer. [Abstract]2025 Jul 2. PMID: 40601842 -
J Ethnopharmacol
Serum metabolomics and 16S rRNA amplicon sequencing reveal the role of puerarin in alleviating bone loss aggravated by antidiabetic agent pioglitazone in type 2 diabetic mice. [Abstract]2024 Nov 29:340:119128. PMID: 39617084 -
Int J Pharm
3D-printed microneedles loaded with madecassoside for periodontal soft tissue regeneration. [Abstract]2025 Apr 12:125569. PMID: 40228613 -
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PLoS Pathog
Combination of bortezomib and venetoclax targets the pro-survival function of LMP-1 and EBNA-3C of Epstein-Barr virus in spontaneous lymphoblastoid cell lines. [Abstract]2024 Sep 26;20(9):e1012250. PMID: 39325843 -
Int Immunopharmacol
Dexmedetomidine attenuates lipopolysaccharide-induced inflammation through macrophageal IL-10 expression following α7 nAchR activation. [Abstract]2022 Aug:109:108920. PMID: 35691275 -
Nanoscale Adv
Dexamethasone-loaded lipid-polymeric nanoparticles to improve therapy for cisplatin-induced sensorineural hearing loss. [Abstract]2025 Nov 21. PMID: 41278499 -
Appl Microbiol Biotechnol
Aspertaichamide B, a new anti-tumor prenylated indole alkaloid from the fungus Aspergillus japonicus TE-739D. [Abstract]2024 Sep 25;108(1):473. PMID: 39320549 -
Lab Invest
Pharmacological inhibition of serine synthesis enhances temozolomide efficacy by decreasing O6-methylguanine DNA methyltransferase (MGMT) expression and reactive oxygen species (ROS)-mediated DNA damage in glioblastoma. [Abstract]2022 Feb;102(2):194-203. PMID: 34625658 -
J Biol Chem
Disruption of lysosomal nutrient sensing scaffold contributes to pathogenesis of a fatal neurodegenerative lysosomal storage disease. [Abstract]2024 Feb;300(2):105641. PMID: 38211816 -
Neurochem Res
3BDO Alleviates Seizures and Improves Cognitive Function by Regulating Autophagy in Pentylenetetrazol (PTZ)-Kindled Epileptic Mice Model. [Abstract]2022 Dec;47(12):3777-3791. PMID: 36243819 -
Reproduction
Blockade of T-type Ca2+ channels disrupts mitochondrial function and follicle development by inhibiting the PI3K/AKT pathway. [Abstract]2026 Mar 15;171(3):xaag028. PMID: 41761584 -
Rapid Commun Mass Spectrom
LC-MS Determination of Trichloroethylene Glutathione Conjugation Metabolites in a Parkinson's Disease Mouse Model. [Abstract]2025 Nov 15;39(21):e10117. PMID: 40734539 -
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bioRxiv
Selective Sensitivity of Ph-like B-ALL to BRG1 Inhibition Reveals a Novel Targeted Therapy Strategy. [Abstract]2025 Jul 11:2025.07.08.661805. PMID: 40672274 -
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Solvent & Solubility
DMSO : 100 mg/mL (250.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (250.00 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
Rats[1]
Fischer-344 rats (10/group/sex) are administered polyethylene glycol 400 (PEG400) by gavage at 1.0, 2.5 or 5.0 mL/kg (1. l, 2.8 and 5.6 g/kg, respectively) body weight/day 5 days/wk for 13 wk. Animals in the control group receive water by gavage (5.0 mL/kg body weight/treatment day). An additional 10 rats/sex/group are assigned to the control and high-dose groups for a 6-wk recovery period. Evaluation of potential renal toxicity is identified as a primary objective[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Hermansky SJ, et al. Effects of polyethylene glycol 400 (PEG 400) following 13 weeks of gavage treatment in Fischer-344 rats. Food Chem Toxicol. 1995 Feb;33(2):139-49. [Content Brief]
[2]. Xiaoqin Wang, et al. Injectable silk-polyethylene glycol hydrogels. Acta Biomater. 2015 Jan;12:51-61. [Content Brief]
[3]. Avital Beig, et al. Striking the Optimal Solubility-Permeability Balance in Oral Formulation Development for Lipophilic Drugs: Maximizing Carbamazepine Blood Levels. Mol Pharm. 2017 Jan 3;14(1):319-327. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.5000 mL | 12.5000 mL | 25.0000 mL | 62.5000 mL |
| 5 mM | 0.5000 mL | 2.5000 mL | 5.0000 mL | 12.5000 mL | |
| 10 mM | 0.2500 mL | 1.2500 mL | 2.5000 mL | 6.2500 mL | |
| 15 mM | 0.1667 mL | 0.8333 mL | 1.6667 mL | 4.1667 mL | |
| 20 mM | 0.1250 mL | 0.6250 mL | 1.2500 mL | 3.1250 mL | |
| 25 mM | 0.1000 mL | 0.5000 mL | 1.0000 mL | 2.5000 mL | |
| 30 mM | 0.0833 mL | 0.4167 mL | 0.8333 mL | 2.0833 mL | |
| 40 mM | 0.0625 mL | 0.3125 mL | 0.6250 mL | 1.5625 mL | |
| 50 mM | 0.0500 mL | 0.2500 mL | 0.5000 mL | 1.2500 mL | |
| 60 mM | 0.0417 mL | 0.2083 mL | 0.4167 mL | 1.0417 mL | |
| 80 mM | 0.0312 mL | 0.1562 mL | 0.3125 mL | 0.7812 mL | |
| 100 mM | 0.0250 mL | 0.1250 mL | 0.2500 mL | 0.6250 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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