Tetracycline hydrochloride

Name: Tetracycline hydrochloride
Brand: MedChemExpress
SKU: HY-B0474
Rating: 5.0 (51 reviews)

Cat. No.: HY-B0474 Purity: 98.66%: Data Sheet
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Tetracycline hydrochloride is a broad-spectrum antibiotic with oral activity. Tetracycline hydrochloride exhibits activity against a wide range of bacteria including gram-positive, gram-negative bacteria, chlamydiae, mycoplasmas and rickettsiae. Tetracycline hydrochloride can be used for the research of infections.

For research use only. We do not sell to patients.

CAS No. : 64-75-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Customer Review

Based on 51 publication(s) in Google Scholar

Other Forms of Tetracycline hydrochloride:

Tetracycline In-stock
Tetracycline hydrochloride (Standard) In-stock

51 Publications Citing Use of MCE Tetracycline hydrochloride

Flow Cytometry

Bio/Physico-chemical Assay

Cell Imaging/Staining

Microbiological Assay

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: iScience. 2025 Nov 17;28(12):114102.; We performed dual RNA-seq on phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophages sub-populations infected with double-fluorescent-engineered H37Ra in vitro. This strain constitutively expressed Emerald (green) and expressed TagRFP (red) only upon Tetracycline hydrochloride induction, enabling discrimination of bacterial viability by fluorescence-activated cell sorting (FACS).

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]; Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12468. [Abstract]; The protein levels of HEXB in HBV replication cell model. Left, Tetracycline was added to inhibit HBV replication in HepAD38 cells. Right, HepG2.2.15 was treated with NAC (1 mM, 12 h) to abolish ROS produced by HBV replication.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: Microorganisms. 2024 Mar 13;12(3):575.; Percentages of E. coli strains showing antimicrobial resistance to different antimicrobial agents. AMP, Ampicillin; CAZ, Ceftazidime; IPM, Imipenem; MRP, Meropenem; GEN, Gentamicin; OFX, Ofloxacin; SXT, Trimethoprim–sulfamethoxazole; TET, Tetracycline; TGC, Tigecycline; CL, Colistin; FFC, Florfenicol.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2023 Apr 17;133(8):e159941. [Abstract]; THP-1–derived macrophages were infected for 72 hours with Mtb strain H37Ra carrying a dual-color reporter that comprises a constitutively green (Emerald) and a Tetracycline-inducible red (TagRFP) fluorescent protein. Tetracycline (500 ng/mL) was added 24 hours before analysis of the live or dead status of the H37Ra strain in macrophages by flow cytometry.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]; Microdilution checkerboard analysis showed that plumbagin restored the susceptibility of tet(X4)-carrying E. coli J53p47EC and K. pneumoniae K12016p47EC to Tetracycline, in comparison to the tet(X4)-negative strains E. coli J53 and K. pneumoniae K12016.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]; Time-killing assays of resistant E. coli DH5α+pAM401-tet(X4) by a combination of Tetracycline (8 μg/mL, 0-24 h) and Plumbagin.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Apr;78:103943. [Abstract]; Synergistic mechanisms of plumbagin in combination with tetracycline. An accelerated TCA cycle was observed after the treatment with the combination of Plumbagin and Tetracycline (16 and 32 μg/mL).

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]; Immunoblotting analysis of MLKL-Flag, tBid-Flag, and Cleaved Caspase-3 expression in cell death-inducible C2C12 cell lines with or without tetracycline treatment. C2C12-Mlkl-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 12 h to induce necroptosis, and C2C12-tBid-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 6 h to induce apoptosis.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]; Representative phase-contrast images of MuSCs cultured in different fractions of NCM after purification. The complete NCM was used as the positive control (NCM input); C2C12-Mlkl-TetON medium without Tetracycline hydrochloride treatment was used as the negative control (Tet-control). Cells were cultured for 48 h. Scale bars, 100 μm.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Res. 2020 Dec;30(12):1063-1077. [Abstract]; Immunoblotting analysis of the expression and extracellular release of TNC upon necroptosis induction at different time points as indicated. C2C12-Mlkl-TetON cells were treated with 1 μg/mL Tetracycline hydrochloride for 12 h to induce necroptosis. Both the whole cell lysates and NCM were harvested and then subjected to immunoblotting analysis using antibodies as indicated.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jul 21;7(17):2001374. [Abstract]; The pEt_20 polymer mitigates resistance to Azithromycin, Gentamicin, Imipenem, and Tetracycline, and reduces their minimum inhibitory concentration (MIC) to sensitive levels or even lower.

: Tetracycline hydrochloride purchased from MedChemExpress. Usage Cited in: MBio. 2019 Aug 27;10(4). pii: e01949-19. [Abstract]; CD157 deficiency impairs macrophage bactericidal capacity. Peritoneal macrophages from WT mice and Cd157 KO mice treated with sCD157 (+sCD157) (5 μg/ml) or not treated with sCD157 were infected with strain H37Ra harboring a dual-color reporter that comprises a constitutively green (Emerald) and a Tetracycline hydrochloride-inducible red (TagRFP) fluorescent protein for 3 days. Tetracycline hydrochloride (500 ng/ml) was added 24 h before flow cytometry. Macrophages were then harvested and fixed with 4% PFA, and the percentage of cells with live M. tuberculosis (MTB) (red) were determined by FACS.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Tetracycline

In Vitro

Tetracycline hydrochloride shows susceptibilfty of V. vulnlflcus strain B3547 with MIC value of 0.5 g/mL^[2].
Tetracycline hydrochloride inhibits the L-amyloid aggregates formation and disassembles the pre-formed fibrils^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Tetracycline can be used in animal modeling to create genetically engineered disease models. The pharmacokinetics of Tetracycline after intraperitoneal administration in broiler chickens exhibit unique distribution and elimination characteristics. Tetracycline distributes widely in the body, with rapid elimination, high clearance, and a large volume of distribution. After oral administration, Tetracycline shows high gastrointestinal absorption and moderate elimination rates. Although the absorption rate of Tetracycline is higher than that of most other tetracycline drugs, its overall drug exposure is relatively low^[4].

Induction of tumor regression based on BCR-ABL gene silencing^[4]

Background

The BCR-ABL gene encodes an abnormal tyrosine kinase (BCR-ABL protein), which leads to uncontrolled cell proliferation. Tetracycline can induce tumor regression in mice by inhibiting BCR-ABL expression through the suppression of the binding between tTA (tetracycline transactivator) and the Tet-OP (Tetracycline Operator) sequence^[4].

Specific Modeling Methods

Mice: nu/nu • female and male • 6-8 weeks old
Administration: 200 μg/ml • po • single dose

Note

1. Tetracycline is administered orally dissolved in drinking water containing 5% sucrose.
2. Mouse tumor model construction: To induce visible tumors, Ba/F3 cells transduced with SIN-p210 (a retrovirus carrying and inducing tetracycline-dependent BCR-ABL gene expression) are subcutaneously injected into nude mice.

Modeling Indicators

Phenotypic observation: The tumors established in the mice completely regress.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

480.90

Formula

C₂₂H₂₅ClN₂O₈

CAS No.

64-75-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O[C@@]1(C(C(C(N)=O)=C2O)=O)[C@@]([C@@H]2N(C)C)([H])C[C@@]([C@](O)(C3=CC=C4)C)([H])C(C(C3=C4O)=O)=C1O.Cl

Structure Classification

Initial Source

Microorganisms

Streptomyces

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (129.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (103.97 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0794 mL	10.3972 mL	20.7943 mL
5 mM	0.4159 mL	2.0794 mL	4.1589 mL
10 mM	0.2079 mL	1.0397 mL	2.0794 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.66%

Select Batch:

Data Sheet (284 KB) SDS (418 KB)

COA (255 KB) HNMR (272 KB) RP-HPLC (255 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Chopra I, et al.Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance.Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 [Content Brief]

[2]. Bowdre JH, et al. Antibiotic efficacy against Vibrio vulnificus in the mouse: superiority of tetracycline. J Pharmacol Exp Ther. 1983 Jun;225(3):595-8. [Content Brief]

[3]. Forloni G, et al. Anti-amyloidogenic activity of tetracyclines: studies in vitro. FEBS Lett. 2001 Jan 5;487(3):404-7. [Content Brief]

[4]. Dugray A, et al. Rapid generation of a tetracycline-inducible BCR-ABL defective retrovirus using a single autoregulatory retroviral cassette. Leukemia. 2001 Oct;15(10):1658-62. [Content Brief]

[5]. Ziółkowski H, et al. Comparative pharmacokinetics of chlortetracycline, tetracycline, minocycline, and tigecycline in broiler chickens. Poult Sci. 2020 Oct;99(10):4750-4757. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.0794 mL	10.3972 mL	20.7943 mL	51.9859 mL
	5 mM	0.4159 mL	2.0794 mL	4.1589 mL	10.3972 mL
	10 mM	0.2079 mL	1.0397 mL	2.0794 mL	5.1986 mL
	15 mM	0.1386 mL	0.6931 mL	1.3863 mL	3.4657 mL
	20 mM	0.1040 mL	0.5199 mL	1.0397 mL	2.5993 mL
	25 mM	0.0832 mL	0.4159 mL	0.8318 mL	2.0794 mL
	30 mM	0.0693 mL	0.3466 mL	0.6931 mL	1.7329 mL
	40 mM	0.0520 mL	0.2599 mL	0.5199 mL	1.2996 mL
	50 mM	0.0416 mL	0.2079 mL	0.4159 mL	1.0397 mL
	60 mM	0.0347 mL	0.1733 mL	0.3466 mL	0.8664 mL
	80 mM	0.0260 mL	0.1300 mL	0.2599 mL	0.6498 mL
	100 mM	0.0208 mL	0.1040 mL	0.2079 mL	0.5199 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.