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  3. Doxycycline

Doxycycline, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline shows antibacterial activity and anti-cancer cell proliferation activity.

For research use only. We do not sell to patients.

Doxycycline Chemical Structure

Doxycycline Chemical Structure

CAS No. : 564-25-0

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Solid + Solvent
10 mM * 1 mL in DMSO
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Customer Review

Based on 66 publication(s) in Google Scholar

Other Forms of Doxycycline:

Top Publications Citing Use of Products

65 Publications Citing Use of MCE Doxycycline

WB

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96.  [Abstract]

    Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Doxycycline, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5].

    IC50 & Target

    Tetracycline

     

    In Vitro

    Doxycycline (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells[2].
    Doxycycline (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: LNT-229, G55, and U343 glioma cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 4 days
    Result: Affected growth of glioma cells only under high concentration (10 µg/mL).

    Cell Viability Assay[2]

    Cell Line: SVG cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 24 hours
    Result: Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

    Cell Proliferation Assay[4]

    Cell Line: MCF 12A, 293T cells
    Concentration: 100 ng/mL, 1 µg/mL
    Incubation Time: 96 hours
    Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

    Cell Viability Assay[5]

    Cell Line: MCF-7, MDA-MB-468 cells
    Concentration: 0-250 μM
    Incubation Time: 72 hours
    Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
    In Vivo

    Doxycycline (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner[3].
    Doxycycline and Tetracycline (HY-A0107), act systemically after absorption from the upper gastrointestinal tract. The main advantage of Doxycycline over Tetracycline is its longer activity, and it can be taken twice or once a day. The peak concentration of both drugs is similar, but in the case of Doxycycline the time to peak concentration is shorter, and half life is significantly longer[6].

    Doxycycline (Dox) is often used as an inducer in molecular biology studies to induce gene expression. In cells or model animals that have constructed tetracycline induced expression systems (Tet-On/Tet-Off systems), the expression of target genes can be precisely controlled by adding or removing Dox[7][8][9][10].

    Dose reference for Dox induction[7][8]:
    (1) Model animal: male Sprague–Dawley rats
    Tet regulatory system:20-3000 ppm of Dox is supplied in diet
    (2) Model animal: Cags mice
    Tet regulatory system:625 ppm of Dox is supplied in diet

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3]
    Dosage: 200 or 800 mg/kg
    Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months
    Result: Reduced active MMP-9 in a dose-dependent manner.
    Clinical Trial
    Molecular Weight

    444.43

    Formula

    C22H24N2O8

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to brown

    SMILES

    O=C(N)C(C1=O)=C([C@H]([C@@]2([C@H]([C@@]3([C@H](C4=C(C(O)=CC=C4)C(C3=C([C@@]21O)O)=O)C)[H])O)[H])N(C)C)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (225.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2501 mL 11.2504 mL 22.5007 mL
    5 mM 0.4500 mL 2.2501 mL 4.5001 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: ≥98.0%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2501 mL 11.2504 mL 22.5007 mL 56.2518 mL
    5 mM 0.4500 mL 2.2501 mL 4.5001 mL 11.2504 mL
    10 mM 0.2250 mL 1.1250 mL 2.2501 mL 5.6252 mL
    15 mM 0.1500 mL 0.7500 mL 1.5000 mL 3.7501 mL
    20 mM 0.1125 mL 0.5625 mL 1.1250 mL 2.8126 mL
    25 mM 0.0900 mL 0.4500 mL 0.9000 mL 2.2501 mL
    30 mM 0.0750 mL 0.3750 mL 0.7500 mL 1.8751 mL
    40 mM 0.0563 mL 0.2813 mL 0.5625 mL 1.4063 mL
    50 mM 0.0450 mL 0.2250 mL 0.4500 mL 1.1250 mL
    60 mM 0.0375 mL 0.1875 mL 0.3750 mL 0.9375 mL
    80 mM 0.0281 mL 0.1406 mL 0.2813 mL 0.7031 mL
    100 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5625 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Doxycycline
    Cat. No.:
    HY-N0565
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