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  4. Doxycycline

Doxycycline, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline shows antibacterial activity and anti-cancer cell proliferation activity.

For research use only. We do not sell to patients.

CAS No. : 564-25-0

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Based on 173 publication(s) in Google Scholar

Other Forms of Doxycycline:

Doxycycline Related Antibodies

Top Publications Citing Use of Products

173 Publications Citing Use of MCE Doxycycline

WB
In Vivo Efficacy Study
IHC
In Vivo Imaging
Cell Imaging/Staining
RT-PCR
ELISA
IF
Microbiological Assay

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 May;45(5):608-631.  [Abstract]

    Bioluminescence imaging of the lung metastasis burden after inoculation of MKN45 cells with DOX (500 mg/kg, daily, 30 days)-induced CagA expression and MA treatment.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 May;45(5):608-631.  [Abstract]

    After inoculating MKN45 cells with DOX-induced CagA expression and MA treatment, HE staining was performed on lung tissue sections of a specified group. The quantitative results of the number of lung metastases are shown on the right.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2025 Apr;27(4):641-653.  [Abstract]

    HeLa cells stably expressing tet-on-shScramble (Scr) or tet-on-shMAT2A were pretreated with Doxycycline hydrochloride (Dox, 1 μg/ mL) for two days, and the cell lysates were analysed by immunoblotting with the indicated antibodies.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Oct 8;524:169421.

    Therapeutic effect of VRC, Doxycycline hydrochloride (Doxy, 0.05 %, 5 μl, 4 times per day for 5 days) on DXMS- associated severe FK. Dynamic phenotype of corneas with different groups observed by slit lamp on each day after infection (n = 6/group).

    Doxycycline purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Mar 19;44(1):99.  [Abstract]

    Nude mice (nu/nu) were surgically engrafted intracranially with GSCs labeled with luciferase and transduced with a Doxycycline hydrochloride (DOX)-inducible system for shPHGDH expression. Mice harboring either GSC19 (A) or GSC29 (B) were randomly assigned to treatment groups (6 mice per group). Starting from day 7 or day 9 post-engraftment, as per the study design (A, B, left), treatments were administered: control, 3 Gy ionizing radiation (IR) delivered weekly over three sessions, 2 mg/mL DOX in the drinking water or a combination of IR and DOX. The progression of GBM xenografts was monitored through bioluminescence imaging, and representative images are displayed (A, B, right).

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Nov 25;44(11):116496.  [Abstract]

    MOLM-13 cells were treated with Doxycycline hydrochloride (25 µM) or Chloramphenicol (25 µM) for 24 h. Mitochondrial translation and MMP were examined (n=3)

    Doxycycline purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141.  [Abstract]

    Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305.  [Abstract]

    RT-qPCR analysis of STAT5A and target gene expression in STB-BL with DOX (5 μM, 6 days)-inducible overexpression of STAT5A.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305.  [Abstract]

    RT-qPCR analysis of MITF and target gene expression in STB-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305.  [Abstract]

    ELISA analysis of CSH2 expression in trophoblast organoids derived from hTSCs-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305.  [Abstract]

    Bright fields and immunofluorescence analysis of trophoblast markers (CDH1 and hCG) in trophoblast organoids derived from hTSCs-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272.  [Abstract]

    The expression of NHE7 in Dox-inducible NHE7-mAID-KO MHCC97L cells with or without doxycycline (DOX, 2 mg/mL) and indole-3-acetic acid (IAA, 50 μg/mL) treatment for 24 h was examined by immunoblotting.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272.  [Abstract]

    Mice orthotopically injected with NHE7-mAID-KO MHCC97L cells were administered vehicle (DMSO), DOX (2 mg/mL in drinking water containing 5% sucrose), IAA (25 mg/kg/day) and sorafenib (30 mg/kg/day) for 2 weeks (n = 6). Bioluminescence imaging of whole mice was performed at the end of the experiment, and the signal intensity was quantified.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272.  [Abstract]

    Mice orthotopically injected with NHE7-mAID-KO MHCC97L cells were administered vehicle (DMSO), DOX (2 mg/mL in drinking water containing 5% sucrose), IAA (25 mg/kg/day) and sorafenib (30 mg/kg/day) for 2 weeks (n = 6). Immunohistochemistry of NHE7 and Rab21 in liver tumor tissues.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2020 Sep 9;19(1):139.  [Abstract]

    ATL cells MT1 stably transfected with a TET-inducible FBXW7 wild type or W425R were induced with doxycycline (2 μg/ml, 24 h) and degradation (WT) or lack thereof (W425R) of endogenous BRAF was confirmed by Western blot.

    Doxycycline purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2020 Sep 9;19(1):139.  [Abstract]

    ATL cells MT1 stably transfected with a TET-inducible FBXW7 wild type, endogenous degradation of BRAF by wild type FBXW7 was confirmed by immunohistochemistry following induction of FBXW7 with doxycycline (2 μg/ml, 24 h).

    Doxycycline purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96.  [Abstract]

    Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Doxycycline, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5].

    IC50 & Target

    Tetracycline

     

    Cellular Effect
    Cell Line Type Value Description References
    HepG2 CC50
    20 μM
    Compound: Doxycycline
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 37857143]
    HepG2 CC50
    20 μM
    Compound: Doxycycline
    Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay
    Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay
    		[PMID: 38452406]
    In Vitro

    Doxycycline (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells[2].
    Doxycycline (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Doxycycline Related Antibodies

    Cell Viability Assay[2]

    Cell Line: LNT-229, G55, and U343 glioma cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 4 days
    Result: Affected growth of glioma cells only under high concentration (10 µg/mL).

    Cell Viability Assay[2]

    Cell Line: SVG cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 24 hours
    Result: Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

    Cell Proliferation Assay[4]

    Cell Line: MCF 12A, 293T cells
    Concentration: 100 ng/mL, 1 µg/mL
    Incubation Time: 96 hours
    Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

    Cell Viability Assay[5]

    Cell Line: MCF-7, MDA-MB-468 cells
    Concentration: 0-250 μM
    Incubation Time: 72 hours
    Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Doxycycline (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner[3].
    Doxycycline and Tetracycline (HY-A0107), act systemically after absorption from the upper gastrointestinal tract. The main advantage of Doxycycline over Tetracycline is its longer activity, and it can be taken twice or once a day. The peak concentration of both agents is similar, but in the case of Doxycycline the time to peak concentration is shorter, and half life is significantly longer[6].

    Doxycycline (Dox) is often used as an inducer in molecular biology studies to induce gene expression. In cells or model animals that have constructed tetracycline induced expression systems (Tet-On/Tet-Off systems), the expression of target genes can be precisely controlled by adding or removing Dox[7][8].
    Dose reference for Dox induction[7][8]:
    (1) Model animal: male Sprague-Dawley rats
    Tet regulatory system: 20-3000 ppm of Dox is supplied in diet.
    (2) Model animal: Cags mice
    Tet regulatory system: 625 ppm of Dox is supplied in diet.
    Induction of Modeling ON-OFF System (Gene expression regulation)[6][7][8]
    Background
    Doxycycline is often used as an inducer in molecular biology research to induce gene expression. In cells or model animals that have constructed a Tetracycline (Tet; HY-A0107) inducible expression (Tet-ON/Tet-OFF) system, the expression of the target gene can be precisely controlled by adding or removing Doxycycline. Doxycycline can act as an inhibitor of transcriptional activation in the Tetracycline (Tc)-controlled transactivation (tTA) system, and as an inducer of transcriptional activation in the "reverse tTA' system. Doxycycline and Tetracycline both act systemically after being absorbed by the upper gastrointestinal tract. In comparison, the main advantage of Doxycycline is that it has a longer activity and can be taken twice or once a day. Although the peak concentrations of the two are similar, Doxycycline takes a shorter time to reach peak concentration and has a significantly longer half-life.
    Specific Modeling Methods
    Rat[8]: Sprague-Dawley rats • male • adult middle-aged (12-month-old)
    Administration: (for GDNF as targeted gene) 3g/kg (dietary with regular food) • po • once daily for 6 days
    Note
    (1) Recommend use the recombinant adeno-associated virus (rAAV)-based bicistronic tetracycline (tet)-OFF construct was used for dynamic control of GDNF (target gene) expression during long-term expression[7].
    (2) 3 g/kg dietary DOX produced DOX serum levels equivalent to 1mg/ml DOX in drinking water.
    Modeling Indicators
    Molecular changes: The expression level of the target gene decreases.
    Phenotype changes: The positively correlated phenotype corresponding to the target gene is alleviated.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3]
    Dosage: 200 or 800 mg/kg
    Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months
    Result: Reduced active MMP-9 in a dose-dependent manner.
    Molecular Weight

    444.43

    Formula

    C22H24N2O8
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to brown

    SMILES

    O=C(N)C(C1=O)=C([C@H]([C@@]2([C@H]([C@@]3([C@H](C4=C(C(O)=CC=C4)C(C3=C([C@@]21O)O)=O)C)[H])O)[H])N(C)C)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (225.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2501 mL 11.2504 mL 22.5007 mL
    5 mM 0.4500 mL 2.2501 mL 4.5001 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (4.68 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.20%

    SDS (419 KB)

    COA (255 KB) HNMR (317 KB) RP-HPLC (248 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2501 mL 11.2504 mL 22.5007 mL 56.2518 mL
    5 mM 0.4500 mL 2.2501 mL 4.5001 mL 11.2504 mL
    10 mM 0.2250 mL 1.1250 mL 2.2501 mL 5.6252 mL
    15 mM 0.1500 mL 0.7500 mL 1.5000 mL 3.7501 mL
    20 mM 0.1125 mL 0.5625 mL 1.1250 mL 2.8126 mL
    25 mM 0.0900 mL 0.4500 mL 0.9000 mL 2.2501 mL
    30 mM 0.0750 mL 0.3750 mL 0.7500 mL 1.8751 mL
    40 mM 0.0563 mL 0.2813 mL 0.5625 mL 1.4063 mL
    50 mM 0.0450 mL 0.2250 mL 0.4500 mL 1.1250 mL
    60 mM 0.0375 mL 0.1875 mL 0.3750 mL 0.9375 mL
    80 mM 0.0281 mL 0.1406 mL 0.2813 mL 0.7031 mL
    100 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5625 mL
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    Doxycycline Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Doxycycline564-25-0MMPBacterialAntibioticParasiteMatrix metalloproteinasesantibacterial activityanti-proliferative activityantibioticLNT-229G55U343SVGMCF 12A293TInhibitorinhibitorinhibit

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