Cat. No.: HY-N0565B

Doxycycline hyclate

(Synonyms: Doxycycline hydrochloride hemiethanolate hemihydrate; WC2031)

182 Cited Publications

Customer Review

Based on 182 publication(s) in Google Scholar

Doxycycline hyclate, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline hyclate shows antibacterial activity and anti-cancer cell proliferation activity. Doxycycline hyclate can be used to construct gene expression regulation models.

For research use only. We do not sell to patients.

Purity: 99.19%
CAS No.: 24390-14-5
Formula: C₂₃H₂₉ClN₂O₉
Molecular Weight:512.94

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

Doxycycline hyclate Chemical Structure

CAS No.: 24390-14-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Induced Disease Models
Chemical Information
Publications (182)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Tetracycline

All Antibiotic Isoforms :

All MMP Isoforms :

All Parasite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 21741131]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 21852132]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 22889559]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 24946216]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells incubated for 72 hrs by MTT assay	[PMID: 25282267]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 25791675]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells measured after 72 hrs by MTT assay	[PMID: 27155463]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 27654395]
HepG2	CC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 34364164]
HepG2	IC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 19482476]
HepG2	IC₅₀	20 μM Compound: doxycycline	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 19926173]
HT-1080	IC₅₀	21.4 μM Compound: 7	Inhibition of cell invasion in human HT-1080 cells for 24 hrs by Calcein AM staining based fluorometric analysis Inhibition of cell invasion in human HT-1080 cells for 24 hrs by Calcein AM staining based fluorometric analysis	[PMID: 35969157]
K562	IC₅₀	15 μM Compound: Doxycycline	Cytotoxicity against human K562 cells after 72 hrs by flow cytometry Cytotoxicity against human K562 cells after 72 hrs by flow cytometry	[PMID: 19482476]
K562	IC₅₀	15 μM Compound: doxycycline	Cytotoxicity against human K562 cells after 72 hrs by flow cytometry Cytotoxicity against human K562 cells after 72 hrs by flow cytometry	[PMID: 19926173]
THP-1	IC₅₀	20 μM Compound: Doxycycline	Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry	[PMID: 19748781]

In Vitro

Doxycycline hyclate (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations^[2].
Doxycycline hyclate (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells^[2].
Doxycycline hyclate (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines^[4].
Doxycycline hyclate (0-250 μM, 72 h) inhibits cell viability of breast cancer cells ^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

LNT-229, G55, and U343 glioma cells
Concentration:

0.01, 0.1, 1 or 10 µg/mL
Incubation Time:

4 days
Result:

Affected growth of glioma cells only under high concentrations (10 µg/mL).

Cell Viability Assay^[2]

Cell Line:

SVG cells
Concentration:

0.01, 0.1, 1 or 10 µg/mL
Incubation Time:

24 hours
Result:

Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

Cell Proliferation Assay^[4]

Cell Line:

MCF 12A, 293T cells
Concentration:

100 ng/mL, 1 µg/mL
Incubation Time:

96 hours
Result:

Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

Cell Viability Assay^[5]

Cell Line:

MCF-7, MDA-MB-468 cells
Concentration:

0-250 μM
Incubation Time:

72 hours
Result:

Inhibited breast cancer cells in a dose-dependent manner with IC₅₀ values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.

In Vivo

Animal Model:

6-month-old female Heterozygous Col3a1-deficient (HT) mice^[3]
Dosage:

200 or 800 mg/kg
Administration:

Oral gavage; 200 or 800 mg/kg; once daily; 3 months
Result:

Reduced MMP-9 activity in a dose-dependent manner.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Doxycycline (oral gavage; 200 or 800 mg/kg; once daily; 3 months) hyclate reduces MMP-9 activity in untreated HT mice in a dose-dependent manner^[3].
Doxycycline hyclate and Tetracycline (HY-A0107), act systemically after absorption from the upper gastrointestinal tract. The main advantage of Doxycycline hyclate over Tetracycline is its longer activity, and it can be taken twice or once a day. The peak concentration of both agents is similar, but in the case of Doxycycline the time to peak concentration is shorter, and half life is significantly longer^[6].

Doxycycline (Dox) hyclate is often used as an inducer in molecular biology studies to induce gene expression. In cells or model animals that have constructed tetracycline induced expression systems (Tet-On/Tet-Off systems), the expression of target genes can be precisely controlled by adding or removing Dox^[7]^[8]^[9]^[10].
Dose reference for Dox induction^[7]^[8]:
(1) Model animal: male Sprague-Dawley rats
Tet regulatory system: 20-3000 ppm of Dox is supplied in diet.
(2) Model animal: Cags mice
Tet regulatory system: 625 ppm of Dox is supplied in diet.

Induction of Modeling ON-OFF System (Gene expression regulation)^[6]^[7]^[8]

Background

Doxycycline hyclate is often used as an inducer in molecular biology research to induce gene expression. In cells or model animals that have constructed a Tetracycline (Tet; HY-A0107) inducible expression (Tet-ON/Tet-OFF) system, the expression of the target gene can be precisely controlled by adding or removing Doxycycline hyclate. Doxycycline hyclate can act as an inhibitor of transcriptional activation in the Tetracycline (Tc)-controlled transactivation (tTA) system, and as an inducer of transcriptional activation in the "reverse tTA' system.
Doxycycline and Tetracycline both act systemically after being absorbed by the upper gastrointestinal tract. In comparison, the main advantage of Doxycycline is that it has a longer activity and can be taken twice or once a day. Although the peak concentrations of the two are similar, Doxycycline takes a shorter time to reach peak concentration and has a significantly longer half-life.

Specific Modeling Methods

Rat^[8]: Sprague-Dawley rats • male • adult middle-aged (12-month-old)
Administration: (for GDNF as targeted gene) 3g/kg (Doxycycline; dietary with regular food) • po • once daily for 6 days

Note

(1) Recommend use the recombinant adeno-associated virus (rAAV)-based bicistronic tetracycline (tet)-OFF construct was used for dynamic control of GDNF (target gene) expression during long-term expression^[7].
(2) 3 g/kg dietary DOX produced DOX serum levels equivalent to 1mg/ml DOX in drinking water.

Modeling Indicators

Molecular changes: The expression level of the target gene decreases.

Phenotype changes: The positively correlated phenotype corresponding to the target gene is alleviated.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 24390-14-5
Appearance Solid
Molecular Weight 512.94
Formula C₂₃H₂₉ClN₂O₉
Color White to yellow
SMILES

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.Cl.[0.5H2O].[0.5C2H6O]
Synonyms

Doxycycline hydrochloride hemiethanolate hemihydrate; WC2031
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

Publications (182)

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Journal Impact Factor

Nature

Ageing promotes metastasis via activation of the integrated stress response. [Abstract]

2026 Apr;652(8112):1339-1348. PMID: 41813904
Cell

Sympathetic-epithelial crosstalk governs tissue-resident memory T cell immunosurveillance in the skin. [Abstract]

2026 Mar 5;189(5):1323-1340.e25. PMID: 41616781
Cell

Extrachromosomal DNA replication and maintenance couple with DNA damage pathway in tumors. [Abstract]

2025 Jun 26;188(13):3405-3421.e27. PMID: 40300601
Cell

Opioid-induced fragile-like regulatory T cells contribute to withdrawal. [Abstract]

2023 Feb 2;186(3):591-606.e23. PMID: 36669483
Mol Cancer

Dying tumor cell-derived exosomal miR-194-5p potentiates survival and repopulation of tumor repopulating cells upon radiotherapy in pancreatic cancer. [Abstract]

2020 Mar 30;19(1):68. PMID: 32228703
Mol Cancer

Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells. [Abstract]

2020 Sep 9;19(1):139. PMID: 32907612

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2020 Sep 9;19(1):139. [Abstract]

ATL cells MT1 stably transfected with a TET-inducible FBXW7 wild type or W425R were induced with doxycycline (2 μg/ml, 24 h) and degradation (WT) or lack thereof (W425R) of endogenous BRAF was confirmed by Western blot.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2020 Sep 9;19(1):139. [Abstract]

ATL cells MT1 stably transfected with a TET-inducible FBXW7 wild type, endogenous degradation of BRAF by wild type FBXW7 was confirmed by immunohistochemistry following induction of FBXW7 with doxycycline (2 μg/ml, 24 h).
Nat Genet

Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy. [Abstract]

2024 Feb;56(2):294-305. PMID: 38267607

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305. [Abstract]

RT-qPCR analysis of STAT5A and target gene expression in STB-BL with DOX (5 μM, 6 days)-inducible overexpression of STAT5A.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305. [Abstract]

RT-qPCR analysis of MITF and target gene expression in STB-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305. [Abstract]

ELISA analysis of CSH2 expression in trophoblast organoids derived from hTSCs-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Nat Genet. 2024 Feb;56(2):294-305. [Abstract]

Bright fields and immunofluorescence analysis of trophoblast markers (CDH1 and hCG) in trophoblast organoids derived from hTSCs-BL with DOX (5 μM, 6 days)-inducible overexpression of MITF.
Adv Mater

Senescence Reprogramming Unleashes Tumor Immune Surveillance via Coordinated Gene Modulation. [Abstract]

2025 Dec 1:e16597. PMID: 41324273
Cancer Commun (Lond)

Helicobacter pylori CagA elevates FTO to induce gastric cancer progression via a "hit-and-run" paradigm. [Abstract]

2025 May;45(5):608-631. PMID: 39960839

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 May;45(5):608-631. [Abstract]

Bioluminescence imaging of the lung metastasis burden after inoculation of MKN45 cells with DOX (500 mg/kg, daily, 30 days)-induced CagA expression and MA treatment.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 May;45(5):608-631. [Abstract]

After inoculating MKN45 cells with DOX-induced CagA expression and MA treatment, HE staining was performed on lung tissue sections of a specified group. The quantitative results of the number of lung metastases are shown on the right.
Cancer Commun (Lond)

NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma. [Abstract]

2024 Feb;44(2):251-272. PMID: 38152992

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272. [Abstract]

The expression of NHE7 in Dox-inducible NHE7-mAID-KO MHCC97L cells with or without doxycycline (DOX, 2 mg/mL) and indole-3-acetic acid (IAA, 50 μg/mL) treatment for 24 h was examined by immunoblotting.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272. [Abstract]

Mice orthotopically injected with NHE7-mAID-KO MHCC97L cells were administered vehicle (DMSO), DOX (2 mg/mL in drinking water containing 5% sucrose), IAA (25 mg/kg/day) and sorafenib (30 mg/kg/day) for 2 weeks (n = 6). Bioluminescence imaging of whole mice was performed at the end of the experiment, and the signal intensity was quantified.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Feb;44(2):251-272. [Abstract]

Mice orthotopically injected with NHE7-mAID-KO MHCC97L cells were administered vehicle (DMSO), DOX (2 mg/mL in drinking water containing 5% sucrose), IAA (25 mg/kg/day) and sorafenib (30 mg/kg/day) for 2 weeks (n = 6). Immunohistochemistry of NHE7 and Rab21 in liver tumor tissues.
Drug Resist Updat

ZBP1 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in ovarian cancer. [Abstract]

2026 Jan:84:101319. PMID: 41192279
Drug Resist Updat

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane. [Abstract]

2024 Jun 24:76:101112. PMID: 38924997
Cell Stem Cell

Kidney organoid models reveal cilium-autophagy metabolic axis as a therapeutic target for PKD both in vitro and in vivo. [Abstract]

2024 Jan 4;31(1):52-70.e8. PMID: 38181751
Nat Microbiol

Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains. [Abstract]

2023 Mar;8(3):410-423. PMID: 36759752
Nat Cell Biol

Peritumoural adipose tissue promotes ferroptosis resistance by 3-hydroxykynurenine-mediated suppression of ferritinophagy. [Abstract]

2026 Apr;28(4):783-796. PMID: 41813885
Nat Cell Biol

TOLLIP targets GSDME-NT-carrying endocytic vesicles for autophagy to regulate pyroptosis and chemotherapy efficacy. [Abstract]

2026 Apr;28(4):812-827. PMID: 41803502
Nat Cell Biol

PRMT5-mediated arginine methylation stabilizes GPX4 to suppress ferroptosis in cancer. [Abstract]

2025 Apr;27(4):641-653. PMID: 40033101

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2025 Apr;27(4):641-653. [Abstract]

HeLa cells stably expressing tet-on-shScramble (Scr) or tet-on-shMAT2A were pretreated with Doxycycline hydrochloride (Dox, 1 μg/ mL) for two days, and the cell lysates were analysed by immunoblotting with the indicated antibodies.
Mol Cell

Targeted citrullination enables p53 binding to non-canonical sites. [Abstract]

2025 Oct 2;85(19):3588-3604.e11. PMID: 41043392
Mol Cell

Lactylation of XLF promotes non-homologous end-joining repair and chemoresistance in cancer. [Abstract]

2025 Jul 17;85(14):2654-2672.e7. PMID: 40680721
Mol Cell

MLKL activity requires a splicing-regulated, druggable intramolecular interaction. [Abstract]

2025 Apr 3:S1097-2765(25)00259-X. PMID: 40209701
Cancer Res

Loss of CDKN2A Enhances the Efficacy of Immunotherapy in EGFR-Mutant Non-Small Cell Lung Cancer. [Abstract]

2025 Feb 1;85(3):585-601. PMID: 39514336
Cancer Res

LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity. [Abstract]

2023 Oct 2;83(19):3220-3236. PMID: 37463119
Nat Protoc

Inducible CRISPR-Cas9 screening platform to interrogate non-proliferative cellular states. [Abstract]

2025 Oct 8. PMID: 41062702
Nat Commun

ELMO2 is a therapeutic vulnerability in mesenchymal-like and drug-resistant non-small cell lung cancer. [Abstract]

2026 Apr 17;17(1):5369. PMID: 41997974
Nat Commun

2026 Apr 13;17(1):5061. PMID: 41974717
Nat Commun

A neurotoxic cryptic peptide arising from TDP-43-dependent cryptic splicing of PKN1. [Abstract]

2026 Feb 20;17(1):2963. PMID: 41720774
Nat Commun

Noncanonical calcium-independent TRPM4 activation governs intestinal fluid homeostasis. [Abstract]

2026 Jan 8;17(1):1253. PMID: 41507162
Nat Commun

Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia. [Abstract]

2025 Jul 1;16(1):5425. PMID: 40593719
Nat Commun

Gadd45g insufficiency drives the pathogenesis of myeloproliferative neoplasms. [Abstract]

2024 Apr 6;15(1):2989. PMID: 38582902
Nat Commun

Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models. [Abstract]

2023 Dec 20;14(1):8461. PMID: 38123537
Cell Death Differ

RIPK3 sequentially recruits MLKL and RIPK1 to induce PANoptosis and chemokine production. [Abstract]

2026 Apr 4. PMID: 41935203
Cell Death Differ

The PLEKHA1-TACC2 fusion gene drives tumorigenesis via vascular mimicry formation in esophageal squamous-cell carcinoma. [Abstract]

2025 Jul 5. PMID: 40615663
Cell Death Differ

NKAP acts with HDAC3 to prevent R-loop associated genome instability. [Abstract]

2023 Jul;30(7):1811-1828. PMID: 37322264
Cell Death Differ

MEK nuclear localization promotes YAP stability via sequestering β-TrCP in KRAS mutant cancer cells. [Abstract]

2019 Nov;26(11):2400-2415. PMID: 30833665
Neuron

Social interaction in mice suppresses breast cancer progression via a corticoamygdala neural circuit. [Abstract]

2025 Jul 29:S0896-6273(25)00509-4. PMID: 40752486
Neuron

The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways. [Abstract]

2025 Jul 23;113(14):2310-2324.e7. PMID: 40345185
Adv Sci (Weinh)

Targeting PRKCN, an Essential Driver Orchestrating mTOR-IRF4 Axis Independently of Kinase Activity, in Multiple Myeloma. [Abstract]

2026 Apr;13(21):e18975. PMID: 41655233
Adv Sci (Weinh)

Faecalibaculum rodentium Alleviates Ionizing Radiation-Induced Damage in Mice by Improving Intestinal Integrity and Hematopoiesis via Its Metabolite Butyrate. [Abstract]

2025 Oct 13:e09383. PMID: 41082369
Adv Sci (Weinh)

USP8-Governed MDA5 Homeostasis Promotes Innate Immunity and Autoimmunity. [Abstract]

2025 Jun 17:e03865. PMID: 40525588
Adv Sci (Weinh)

HACD2 Promotes Pancreatic Cancer Progression by Enhancing PKM2 Dissociation From PRKN in a Dehydratase-Independent Manner. [Abstract]

2025 Jan 21:e2407942. PMID: 39836601
Adv Sci (Weinh)

ASH1L in Hepatoma Cells and Hepatic Stellate Cells Promotes Fibrosis-Associated Hepatocellular Carcinoma by Modulating Tumor-Associated Macrophages. [Abstract]

2024 Dec;11(45):e2404756. PMID: 39377228
Adv Sci (Weinh)

A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells. [Abstract]

2022 Aug;9(22):e2104823. PMID: 35652200
Adv Sci (Weinh)

Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells. [Abstract]

2021 Mar 15;8(10):2004344. PMID: 34026451
J Clin Invest

Targeting mitochondrial dynamics of morphine-responsive dopaminergic neurons ameliorates opiate withdrawal. [Abstract]

2024 Jan 18;134(5):e171995. PMID: 38236644
Theranostics

Shh and Olig2 sequentially regulate oligodendrocyte differentiation from hiPSCs for the treatment of ischemic stroke. [Abstract]

2022 Mar 28;12(7):3131-3149. PMID: 35547747
Theranostics

Antibiotic Azithromycin inhibits brown/beige fat functionality and promotes obesity in human and rodents. [Abstract]

2022 Jan 1;12(3):1187-1203. PMID: 35154482
Chem Eng J

2025 Oct 8;524:169421.

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Oct 8;524:169421.

Therapeutic effect of VRC, Doxycycline hydrochloride (Doxy, 0.05 %, 5 μl, 4 times per day for 5 days) on DXMS- associated severe FK. Dynamic phenotype of corneas with different groups observed by slit lamp on each day after infection (n = 6/group).
Nucleic Acids Res

ALKBH5 governs human endoderm fate by regulating the DKK1/4-mediated Wnt/β-catenin activation. [Abstract]

2024 Aug 21:gkae707. PMID: 39166492
Protein Cell

Phase separation of DUX family proteins drives totipotent-like state via 3D genome reorganization and retrotransposon activation. [Abstract]

2026 Mar 14:pwag014. PMID: 41832971
J Exp Clin Cancer Res

PHGDH activation fuels glioblastoma progression and radioresistance via serine synthesis pathway. [Abstract]

2025 Mar 19;44(1):99. PMID: 40102981

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Mar 19;44(1):99. [Abstract]

Nude mice (nu/nu) were surgically engrafted intracranially with GSCs labeled with luciferase and transduced with a Doxycycline hydrochloride (DOX)-inducible system for shPHGDH expression. Mice harboring either GSC19 (A) or GSC29 (B) were randomly assigned to treatment groups (6 mice per group). Starting from day 7 or day 9 post-engraftment, as per the study design (A, B, left), treatments were administered: control, 3 Gy ionizing radiation (IR) delivered weekly over three sessions, 2 mg/mL DOX in the drinking water or a combination of IR and DOX. The progression of GBM xenografts was monitored through bioluminescence imaging, and representative images are displayed (A, B, right).
Sci Adv

Epstein-Barr virus-driven cardiolipin synthesis sustains metabolic remodeling during B cell transformation. [Abstract]

2025 Jan 31;11(5):eadr8837. PMID: 39879311
Cell Discov

Bacterial toxins induce non-canonical migracytosis to aggravate acute inflammation. [Abstract]

2024 Nov 5;10(1):112. PMID: 39500876
Sci Adv

RIPK3 deficiency blocks R-2-hydroxyglutarate-induced necroptosis in IDH-mutated AML cells. [Abstract]

2024 Apr 19;10(16):eadi1782. PMID: 38630819
Sci Adv

Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity. [Abstract]

2022 Jan 28;8(4):eabl5220. PMID: 35080973
Water Res

Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]

2023 Jul 15:240:120110. PMID: 37247434
Redox Biol

Targeting PKLR/MYCN/ROMO1 signaling suppresses neuroendocrine differentiation of castration-resistant prostate cancer. [Abstract]

2023 Jun:62:102686. PMID: 36963289
J Control Release

Hypoxia-responsive Azocalixarene-doxycycline host-guest complex for synergistic myopia control. [Abstract]

2025 Dec 29:390:114586. PMID: 41475499
Cancer Lett

Dysregulation of the ALDH1A3/PML-RARα axis promotes the progression of acute promyelocytic leukemia. [Abstract]

2026 Apr 1:642:218280. PMID: 41580016
J Neuroinflammation

MMPs and NETs are detrimental in CNS-tuberculosis with MMP Inhibition in CNS-tuberculosis mice improving survival. [Abstract]

2025 Oct 11;22(1):230. PMID: 41076519
Cancer Lett

An integrin beta4-EGFR unit promotes hepatocellular carcinoma lung metastases by enhancing anchorage independence through activation of FAK-AKT pathway. [Abstract]

2016 Jun 28;376(1):188-96. PMID: 26996299

Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96. [Abstract]

Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.
Cell Death Dis

Both direct and indirect suppression of MCL1 synergizes with BCLXL inhibition in preclinical models of gastric cancer. [Abstract]

2025 Mar 12;16(1):170. PMID: 40075071
Cell Death Dis

Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2. [Abstract]

2022 Feb 10;13(2):138. PMID: 35145081
Cell Death Dis

Mitochondrial quality control protects photoreceptors against oxidative stress in the H2O2-induced models of retinal degeneration diseases. [Abstract]

2021 Apr 20;12(5):413. PMID: 33879768
Cell Death Dis

SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway. [Abstract]

2020 May 7;11(5):331. PMID: 32382038
Cell Death Dis

FTO is required for myogenesis by positively regulating mTOR-PGC-1α pathway-mediated mitochondria biogenesis. [Abstract]

2017 Mar 23;8(3):e2702. PMID: 28333151
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Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (243.69 mM; Need ultrasonic)

DMSO : 50 mg/mL (97.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9495 mL

9.7477 mL

19.4955 mL

5 mM

0.3899 mL

1.9495 mL

3.8991 mL

10 mM

0.1950 mL

0.9748 mL

1.9495 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline

Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

Protocol 2

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)

Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 20 mg/mL (38.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
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g
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μL
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (295 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Eusebio Manchado, et al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016 Jun 30;534(7609):647-51. [Content Brief]

[2]. Anna-Luisa Luger, et al. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5):1504. [Content Brief]

[3]. Wilfried Briest, et al. Doxycycline ameliorates the susceptibility to aortic lesions in a mouse model for the vascular type of Ehlers-Danlos syndrome. J Pharmacol Exp Ther. 2011 Jun;337(3):621-7. [Content Brief]

[4]. Ethan Ahler, et al. Doxycycline alters metabolism and proliferation of human cell lines. PLoS One. 2013 May 31;8(5):e64561. [Content Brief]

[5]. Le Zhang, et al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745. [Content Brief]

[6]. Manfredsson FP, et al. Tight Long-term dynamic doxycycline responsive nigrostriatal GDNF using a single rAAV vector. Mol Ther. 2009 Nov;17(11):1857-67. [Content Brief]

[7]. Kistner A, et al. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10933-8. [Content Brief]

[8]. Niv Y. Doxycycline in Eradication Therapy of Helicobacter pylori--a Systematic Review and Meta-Analysis. Digestion. 2016;93(2):167-73. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	1.9495 mL	9.7477 mL	19.4955 mL	48.7386 mL
	5 mM	0.3899 mL	1.9495 mL	3.8991 mL	9.7477 mL
	10 mM	0.1950 mL	0.9748 mL	1.9495 mL	4.8739 mL
	15 mM	0.1300 mL	0.6498 mL	1.2997 mL	3.2492 mL
	20 mM	0.0975 mL	0.4874 mL	0.9748 mL	2.4369 mL
	25 mM	0.0780 mL	0.3899 mL	0.7798 mL	1.9495 mL
	30 mM	0.0650 mL	0.3249 mL	0.6498 mL	1.6246 mL
	40 mM	0.0487 mL	0.2437 mL	0.4874 mL	1.2185 mL
	50 mM	0.0390 mL	0.1950 mL	0.3899 mL	0.9748 mL
	60 mM	0.0325 mL	0.1625 mL	0.3249 mL	0.8123 mL
	80 mM	0.0244 mL	0.1218 mL	0.2437 mL	0.6092 mL
H₂O	100 mM	0.0195 mL	0.0975 mL	0.1950 mL	0.4874 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Doxycycline hyclate

182 Cited Publications

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Doxycycline hyclate

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Publications Citing Use of MedChemExpress (MCE) Doxycycline hyclate

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Cat. No.: HY-N0565B