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  3. Doxycycline hyclate

Doxycycline hyclate  (Synonyms: Doxycycline hydrochloride hemiethanolate hemihydrate; WC2031)

Cat. No.: HY-N0565B Purity: 99.19%
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Doxycycline hyclate, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline hyclate shows antibacterial activity and anti-cancer cell proliferation activity.

For research use only. We do not sell to patients.

Doxycycline hyclate Chemical Structure

Doxycycline hyclate Chemical Structure

CAS No. : 24390-14-5

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Solid + Solvent
10 mM * 1 mL in DMSO
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Customer Review

Based on 66 publication(s) in Google Scholar

Other Forms of Doxycycline hyclate:

Top Publications Citing Use of Products

65 Publications Citing Use of MCE Doxycycline hyclate

WB

    Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96.  [Abstract]

    Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Doxycycline hyclate, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline hyclate shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5].

    IC50 & Target

    Tetracycline

     

    In Vitro

    Doxycycline hyclate (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline hyclate (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells[2].
    Doxycycline hyclate (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline hyclate (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: LNT-229, G55, and U343 glioma cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 4 days
    Result: Affected growth of glioma cells only under high concentrations (10 µg/mL).

    Cell Viability Assay[2]

    Cell Line: SVG cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 24 hours
    Result: Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

    Cell Proliferation Assay[4]

    Cell Line: MCF 12A, 293T cells
    Concentration: 100 ng/mL, 1 µg/mL
    Incubation Time: 96 hours
    Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

    Cell Viability Assay[5]

    Cell Line: MCF-7, MDA-MB-468 cells
    Concentration: 0-250 μM
    Incubation Time: 72 hours
    Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
    In Vivo

    Doxycycline (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner[3].
    Doxycycline and Tetracycline (HY-A0107), act systemically after absorption from the upper gastrointestinal tract. The main advantage of Doxycycline over Tetracycline is its longer activity, and it can be taken twice or once a day. The peak concentration of both drugs is similar, but in the case of Doxycycline the time to peak concentration is shorter, and half life is significantly longer[6].

    Doxycycline (Dox) is often used as an inducer in molecular biology studies to induce gene expression. In cells or model animals that have constructed tetracycline induced expression systems (Tet-On/Tet-Off systems), the expression of target genes can be precisely controlled by adding or removing Dox[7][8][9][10].

    Dose reference for Dox induction[7][8][9][10]:
    (1) Model animal: male Sprague–Dawley rats
    Tet regulatory system:20-3000 ppm of Dox is supplied in diet
    (2) Model animal: Cags mice
    Tet regulatory system:625 ppm of Dox is supplied in diet
    (3) Model animal: Transgenic Wistar rats
    Tet regulatory system:2 mg/mL of Dox is supplied in drinking water
    (4) Model animal: Transgenic NRMI inbred mice
    Tet regulatory system:2 mg/mL of Dox is supplied in drinking water

    Dissolution method of Dox[9][10]:
    (1) Prepare Dox working solution
    Dissolve 100 mg Dox into 50 mL drinking water, add 5% sucrose or 2% saccharin to mask the bitter taste, and refresh the water every three days.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3]
    Dosage: 200 or 800 mg/kg
    Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months
    Result: Reduced MMP-9 activity in a dose-dependent manner.
    Clinical Trial
    Molecular Weight

    512.94

    Formula

    C23H29ClN2O9

    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.Cl.[0.5H2O].[0.5C2H6O]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 240 mg/mL (467.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 125 mg/mL (243.69 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9495 mL 9.7477 mL 19.4955 mL
    5 mM 0.3899 mL 1.9495 mL 3.8991 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

    • Protocol 2

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 20 mg/mL (38.99 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.19%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.9495 mL 9.7477 mL 19.4955 mL 48.7386 mL
    5 mM 0.3899 mL 1.9495 mL 3.8991 mL 9.7477 mL
    10 mM 0.1950 mL 0.9748 mL 1.9495 mL 4.8739 mL
    15 mM 0.1300 mL 0.6498 mL 1.2997 mL 3.2492 mL
    20 mM 0.0975 mL 0.4874 mL 0.9748 mL 2.4369 mL
    25 mM 0.0780 mL 0.3899 mL 0.7798 mL 1.9495 mL
    30 mM 0.0650 mL 0.3249 mL 0.6498 mL 1.6246 mL
    40 mM 0.0487 mL 0.2437 mL 0.4874 mL 1.2185 mL
    50 mM 0.0390 mL 0.1950 mL 0.3899 mL 0.9748 mL
    60 mM 0.0325 mL 0.1625 mL 0.3249 mL 0.8123 mL
    80 mM 0.0244 mL 0.1218 mL 0.2437 mL 0.6092 mL
    100 mM 0.0195 mL 0.0975 mL 0.1950 mL 0.4874 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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