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  4. Doxycycline hyclate

Doxycycline hyclate  (Synonyms: Doxycycline hydrochloride hemiethanolate hemihydrate; WC2031)

Cat. No.: HY-N0565B Purity: 99.19%
COA Handling Instructions

Doxycycline hyclate, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor. Doxycycline hyclate shows antibacterial activity and anti-cancer cell proliferation activity.

For research use only. We do not sell to patients.

Doxycycline hyclate Chemical Structure

Doxycycline hyclate Chemical Structure

CAS No. : 24390-14-5

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10 mM * 1 mL in DMSO
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Customer Review

Based on 56 publication(s) in Google Scholar

Other Forms of Doxycycline hyclate:

Doxycycline hyclate Related Antibodies

Top Publications Citing Use of Products

55 Publications Citing Use of MCE Doxycycline hyclate

WB

    Doxycycline hyclate purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2016 Jun 28;376(1):188-96.  [Abstract]

    Doxycycline inducible shRNA can effectivly knockdown the expression of AKT in HCC cells. Cells are treated with 100ng/mL Doxcycline and cultured for the indicated times.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Doxycycline hyclate, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline hyclate shows antibacterial activity and anti-cancer cell proliferation activity[1][2][3][4][5].

    IC50 & Target

    Tetracycline

     

    In Vitro

    Doxycycline hyclate (0.01-10 µg/mL, 4 d) affects growth of glioma cells only under high concentrations[2].
    Doxycycline hyclate (0.01-10 µg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells[2].
    Doxycycline hyclate (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[4].
    Doxycycline hyclate (0-250 μM, 72 h) inhibits cell viability of breast cancer cells [5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Doxycycline hyclate Related Antibodies

    Cell Viability Assay[2]

    Cell Line: LNT-229, G55, and U343 glioma cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 4 days
    Result: Affected growth of glioma cells only under high concentrations (10 µg/mL).

    Cell Viability Assay[2]

    Cell Line: SVG cells
    Concentration: 0.01, 0.1, 1 or 10 µg/mL
    Incubation Time: 24 hours
    Result: Decreaseed MT-CO1 protein content with concentrations of 1 µg/mL and higher.

    Cell Proliferation Assay[4]

    Cell Line: MCF 12A, 293T cells
    Concentration: 100 ng/mL, 1 µg/mL
    Incubation Time: 96 hours
    Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 µg/mL.

    Cell Viability Assay[5]

    Cell Line: MCF-7, MDA-MB-468 cells
    Concentration: 0-250 μM
    Incubation Time: 72 hours
    Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
    In Vivo

    Doxycycline hyclate (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice[3]
    Dosage: 200 or 800 mg/kg
    Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months
    Result: Reduced MMP-9 activity in a dose-dependent manner.
    Clinical Trial
    Molecular Weight

    512.94

    Appearance

    Solid

    Formula

    C23H29ClN2O9
    CAS No.
    SMILES

    O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.Cl.[0.5H2O].[0.5C2H6O]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 years; -20°C, 6 months (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 240 mg/mL (467.89 mM; Need ultrasonic)

    H2O : 125 mg/mL (243.69 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9495 mL 9.7477 mL 19.4955 mL
    5 mM 0.3899 mL 1.9495 mL 3.8991 mL
    10 mM 0.1950 mL 0.9748 mL 1.9495 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 20 mg/mL (38.99 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

    • 3.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3 mg/mL (5.85 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.19%

    COA (262 KB) LCMS (131 KB)

    Handling Instructions (2659 KB)
    References
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    Doxycycline hyclate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    × = ×
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    Keywords:

    Doxycycline hyclate24390-14-5Doxycycline hydrochloride hemiethanolate hemihydrate WC2031WC2031WC 2031WC-2031AntibioticMMPBacterialParasiteMatrix metalloproteinasesantibacterial activityanti-proliferative activityantibioticLNT-229G55U343SVGMCF 12A293TInhibitorinhibitorinhibit

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