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Hexadimethrine bromide (Synonyms: 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide)

Cat. No.: HY-112735 Purity: >99.0%
Handling Instructions

Hexadimethrine bromide is a cationic polymer discovered to enhance retroviral transduction.

For research use only. We do not sell to patients.

Hexadimethrine bromide Chemical Structure

Hexadimethrine bromide Chemical Structure

CAS No. : 28728-55-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in Water USD 55 In-stock
Estimated Time of Arrival: December 31
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Hexadimethrine bromide is a cationic polymer discovered to enhance retroviral transduction.

In Vitro

Hexadimethrine bromide inhibits human mesenchymal stem cell proliferation during lentiviral transduction. Hexadimethrine bromide is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types at concentrations greater than 10 µg/mL. Trypsinized cells exposed to Hexadimethrine bromide are visibly larger in size when viewed under the microscope[1].

Solvent & Solubility
In Vitro: 

H2O : ≥ 125 mg/mL (309.20 mM)

DMSO : 5 mg/mL (12.37 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4736 mL 12.3680 mL 24.7359 mL
5 mM 0.4947 mL 2.4736 mL 4.9472 mL
10 mM 0.2474 mL 1.2368 mL 2.4736 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: 0.5 mg/mL (1.24 mM); Clear solution; Need warming

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

References
Cell Assay
[1]

Trypsinized cells are washed and resuspended in medium at 2×104 cells/mL with or without rhFGF-2 (final concentration=10 ng/mL, PeproTech) and with or without Hexadimethrine bromide at a final Hexadimethrine bromide concentration of 1, 4, or 8 µg/mL. The different conditions are seeded in 96-well plates at 1×103 cells in 50 µL per well in triplicate and cultured at 37°C, 5% CO2. After 6, 9, or 24 hr, the medium is changed and subsequent medium changes occurred every 3-4 days with 50 µL of ± FGF-2 medium (10 ng/mL). Plates are harvested on day 4, 7, 14, and 21 by removing the medium and placing the plates in the -80°C freezer until the day of analysis. The CyQUANT assay is then performed on the wells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

404.27

Formula

C₁₅H₃₆Br₂N₂

CAS No.

28728-55-4

SMILES

C[N+](CCCCCCC)(C)CCC[N+](C)(C)C.[Br-].[Br-]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

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Product Name:
Hexadimethrine bromide
Cat. No.:
HY-112735
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Hexadimethrine bromide

Cat. No.: HY-112735