1. Anti-infection
  2. Bacterial

G-418 disulfate (Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)

Cat. No.: HY-17561 Purity: >98.0%
Data Sheet SDS Handling Instructions

G-418 (disulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, which blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

For research use only. We do not sell to patients.
G-418 disulfate Chemical Structure

G-418 disulfate Chemical Structure

CAS No. : 108321-42-2

Size Price Stock Quantity
10 mM * 1 mL in Water $55 In-stock
1 g $50 In-stock
5 g $160 In-stock
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

G-418 (disulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, which blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

In Vitro

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/mL. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells[1]. In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks[2].

In Vivo

G418 (40 and 80 mg/kg) for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice[3].

References
Animal Administration
[3]

To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

692.71

Formula

C₂₀H₄₄N₄O₁₈S₂

CAS No.

108321-42-2

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

H2O: ≥ 30 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: >98.0%

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Product Name:
G-418 disulfate
Cat. No.:
HY-17561
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