2. Academic Validation
  3. Alpinia hainanensis Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and Evaluation

Alpinia hainanensis Rhizome Extract Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis: Active Ingredient Investigation and Evaluation

  • J Agric Food Chem. 2022 Apr 6;70(13):3989-3999. doi: 10.1021/acs.jafc.1c08038.
Kai-Long Ji 1,  Meng-Zhen Wu 2 3,  Chao-Ying Huang 2 4,  Pian-Chou GongPan 1,  Peng Sun 1,  Yi-Li Sun 2 5,  Jia Li 2 3 4 5 6,  Chun-Fen Xiao 1,  You-Kai Xu 1,  Qing-Fei Fan 1,  Hua-Bin Hu 1,  Qi-Shi Song 1
Affiliations

Affiliations

  • 1 CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, No. 88, Xuefu Rd., Kunming 650223, Yunnan, China.
  • 2 National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
  • 3 School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China.
  • 4 School of Medicine, Shanghai University, Shanghai 200444, China.
  • 5 Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264000, Shandong, China.
  • 6 School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, Zhejiang, China.
PMID: 35321548 DOI: 10.1021/acs.jafc.1c08038
Abstract

Alpinia hainanensis is an important food spice and ethnic medicine in Southwest China. In this study, we found that the EtOAc-soluble fraction (AHE) of the A. hainanensis rhizome ethanol extract could ameliorate dextran sulfate sodium-induced ulcerative colitis (UC). To explore active constituents, five pairs of previously unreported enantiomers (1-5), together with nine known ones (6-14), were obtained. Structural characterization was achieved by comprehensive spectroscopic methods. Compounds 1 and 2 were new curcumin-butyrovanillone hybrids featuring a rare structural fragment of 2,3-dihyrofuran. The anti-inflammatory activities of isolates were evaluated, and the results indicated that compounds (-)-1, (-)-3, 6, 9, 11, and 12 significantly inhibited the nuclear factor-κB signaling pathway. These findings indicate the major active fraction of the A. hainanensis rhizome ethanol extract enriched with diarylheptanoids, Flavonoids, phenolics, and their hybrid mixtures, which could be developed as a nutritional and dietary supplement for treating UC.

Keywords

Alpinia hainanensis; NF-κB signaling pathway; anti-inflammatory effect; curcumin−butyrovanillone hybrids; ulcerative colitis.

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