HMGCR Antibody (YA3518)(PBS only)

Cat. No.: HY-P81197A: Data Sheet User Guide for Antibodies
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HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

Host

Rabbit

분자량

Predicted band size: 97 kDa;

Observed band size: 97 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P04035

Gene ID

3156 [NCBI]

Immunogen

A synthetic peptide of human HMGCR.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IP IP: Immunoprecipitation	1:10-1:100

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:21357570, PubMed:2991281, PubMed:36745799, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668, PubMed:36745799)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Peroxisome membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland; Detected in the cerebellum, fetal brain, testis and adrenal gland; Low abundance except in skin, esophagus, and uterine cervix

Isoforms & Post-Translational Modification：P04035 has 3 isomers: P04035-1: 97476 Da (predicted); P04035-2: 92021 Da (predicted); P04035-3: 99752 Da (predicted).
N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner;Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD) (PubMed:12535518, PubMed:19458199, PubMed:21778231). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2 (PubMed:12535518, PubMed:19458199, PubMed:21778231, PubMed:22143767). The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase (PubMed:12535518, PubMed:19458199, PubMed:21778231). The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97 (PubMed:12535518, PubMed:19458199, PubMed:21778231). The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase (PubMed:22143767). Lys-248 is the main site of ubiquitination (PubMed:19458199). Ubiquitination is enhanced by the presence of a geranylgeranylated protein (PubMed:21778231);Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity

Subunit：Homotetramer (PubMed:10698924). Homodimer (PubMed:10698924). Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway (PubMed:12535518, PubMed:19458199). Interacts with UBIAD1 (PubMed:23169578)

Synonyms

3 hydroxy 3 methylglutaryl CoA reductase antibody; 3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody; 3 hydroxymethylglutaryl CoA reductase antibody; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody; 3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody; 3H3M antibody; HMDH_HUMAN antibody; HMG CoA reductase antibody; HMG CoAR antibody; HMG-CoA reductase antibody; Hmgcr antibody; Hydroxymethylglutaryl CoA reductase antibody; LDLCQ3 antibody; MGC103269 antibody; Red antibody;

각종 서류

User Guide for Antibodies (1077 KB)