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HMGCR Antibody (YA3518)(PBS only)

(Synonyms: 3 hydroxy 3 methylglutaryl CoA reductase antibody; 3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody; 3 hydroxymethylglutaryl CoA reductase antibody; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody; 3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody; 3H3M antibody; HMDH_HUMAN antibody; HMG CoA reductase antibody; HMG CoAR antibody; HMG-CoA reductase antibody; Hmgcr antibody; Hydroxymethylglutaryl CoA reductase antibody; LDLCQ3 antibody; MGC103269 antibody; Red antibody; )

HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HMGCR Antibody (YA3518)(PBS only)
  • HMGCR Antibody (YA3518)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:10-1:100

Product Details

Description

HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 97 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Immunogen

A synthetic peptide of human HMGCR.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HMGCR Antibody (YA3518)(PBS only)
    Western blot analysis was performed on extracts from Caco-2 (lane 1, 20 μg), K562 (lane 2, 20 μg), and HeLa (lane 3, 20 μg) using HMGCR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    HMGCR catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs[1][2][3][4][5][6].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Peroxisome membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland; Detected in the cerebellum, fetal brain, testis and adrenal gland; Low abundance except in skin, esophagus, and uterine cervix

  • Isoforms & Post-Translational Modification

    P04035 has 3 isomers: P04035-1: 97476 Da (predicted); P04035-2: 92021 Da (predicted); P04035-3: 99752 Da (predicted).
    N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner;Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD) (PubMed:12535518, PubMed:19458199, PubMed:21778231). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2 (PubMed:12535518, PubMed:19458199, PubMed:21778231, PubMed:22143767). The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase (PubMed:12535518, PubMed:19458199, PubMed:21778231). The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97 (PubMed:12535518, PubMed:19458199, PubMed:21778231). The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase (PubMed:22143767). Lys-248 is the main site of ubiquitination (PubMed:19458199). Ubiquitination is enhanced by the presence of a geranylgeranylated protein (PubMed:21778231);Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity

  • Subunit

    Homotetramer (PubMed:10698924). Homodimer (PubMed:10698924). Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway (PubMed:12535518, PubMed:19458199). Interacts with UBIAD1 (PubMed:23169578)

  • SwissProt ID

    P04035

  • Gene ID
    3156 [NCBI]
  • Synonyms

    3 hydroxy 3 methylglutaryl CoA reductase antibody; 3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody; 3 hydroxymethylglutaryl CoA reductase antibody; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody; 3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody; 3H3M antibody; HMDH_HUMAN antibody; HMG CoA reductase antibody; HMG CoAR antibody; HMG-CoA reductase antibody; Hmgcr antibody; Hydroxymethylglutaryl CoA reductase antibody; LDLCQ3 antibody; MGC103269 antibody; Red antibody;

References

[1]. Cuccioloni M, et al. Epigallocatechin-3-gallate potently inhibits the in vitro activity of hydroxy-3-methyl-glutaryl-CoA reductase. J Lipid Res. 2011 May;52(5):897-907. [Content Brief]

[2]. Luskey KL, et al. Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved domains responsible for catalytic activity and sterol-regulated degradation. J Biol Chem. 1985 Aug 25;260(18):10271-7. [Content Brief]

[3]. Yogev Y, et al. Limb girdle muscular disease caused by HMGCR mutation and statin myopathy treatable with mevalonolactone. Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2217831120. [Content Brief]

[4]. Brown MS, et al. Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth. J Lipid Res. 1980 Jul;21(5):505-17. [Content Brief]

[5]. Istvan ES, et al. Structural mechanism for statin inhibition of HMG-CoA reductase. Science. 2001 May 11;292(5519):1160-4. [Content Brief]

[6]. Sarver RW, et al. Thermodynamic and structure guided design of statin based inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Med Chem. 2008 Jul 10;51(13):3804-13. [Content Brief]

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HMGCR Antibody (YA3518)(PBS only) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Caco-2 (lane 1, 20 μg), K562 (lane 2, 20 μg), and HeLa (lane 3, 20 μg) using HMGCR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • HMGCR Antibody (YA3518)(PBS only) Product Image
    1/16
  • Experimental Validation Results for HMGCR Antibody (YA3518)(PBS only)
  • HMGCR Antibody (YA3518)(PBS only) Product Image
  • Experimental Validation Results for HMGCR Antibody (YA3518)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Caco-2 (lane 1, 20 μg), K562 (lane 2, 20 μg), and HeLa (lane 3, 20 μg) using HMGCR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for HMGCR Antibody (YA3518)(PBS only)
  • HMGCR Antibody (YA3518)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Caco-2 (lane 1, 20 μg), K562 (lane 2, 20 μg), and HeLa (lane 3, 20 μg) using HMGCR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • HMGCR Antibody (YA3518)(PBS only) Product Image WB