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  5. SMARCA5 Antibody (YA2013)(PBS only)

SMARCA5 Antibody (YA2013)(PBS only)

Cat. No.: HY-P82268A
User Guide for Antibodies Technical Support

SMARCA5 Antibody (YA2013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMARCA5.

For research use only. We do not sell to patients.

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50 μL   Get quote  
100 μL   Get quote  

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

SMARCA5 Antibody (YA2013) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMARCA5.
Host

Rabbit
Molecular Weight
Predicted band size: 122 kDa;
Observed band size: 122 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human SNF2H
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Purity Affinity Purified Conjugation Non-conjugated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：ATPase that possesses intrinsic ATP-dependent nucleosome-remodeling activity (PubMed:12972596, PubMed:28801535). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair; this may require intact histone H4 tails (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:12972596, PubMed:23911928, PubMed:28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed:28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed:28801535). The BAZ1A/ACF1-, BAZ1B/WSTF-, BAZ2A/TIP5- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner (PubMed:14759371, PubMed:15543136, PubMed:28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed:28801535). Binds to core histones together with RSF1, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed:12972596). Involved in DNA replication and together with BAZ1A/ACF1 is required for replication of pericentric heterochromatin in S-phase (PubMed:12434153). Probably plays a role in repression of RNA polymerase I dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter (By similarity). Essential component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex), a chromatin-remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure (PubMed:11980720, PubMed:15543136). The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription (By similarity). Within the B-WICH complex has a role in RNA polymerase III transcription (PubMed:16603771). Mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes (By similarity). Essential component of NoRC-5 ISWI chromatin-remodeling complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing (By similarity)
Subcellular Localization：Nucleus; Chromosome
Expression：
Tissue_specificity:Ubiquitously expressed
Subunit：Component of the ACF-5 ISWI chromatin-remodeling complex (also called the ACF/WCRF complex) at least composed of SMARCA5/SNF2H and BAZ1A/ACF1, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:28801535). Within the complex interacts with BAZ1A/ACF1; the interaction is direct and is required to slide nucleosomes from end to center positions on a DNA template in an ATP-dependent manner (PubMed:10880450, PubMed:12198550, PubMed:12434153, PubMed:28801535). Component of the CHRAC ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity (PubMed:10880450, PubMed:12434153). Within the complex interacts with BAZ1A/ACF1; the interaction is direct and promotes the interaction with the POLE3-CHRAC1 heterodimer (PubMed:10880450, PubMed:12434153, PubMed:14759371). Within the complex interacts with the POLE3-CHRAC1 heterodimer; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction (PubMed:10880450, PubMed:14759371). Neither POLE3 nor CHRAC1 enhances nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed:14759371). Component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex) at least composed of SMARCA5/SNF2H and BAZ1B/WSTF, which regulates the spacing of histone octamers on the DNA template to facilitate access to DNA (PubMed:11980720, PubMed:28801535). Within the complex interacts with BAZ1B/WSTF (PubMed:11980720, PubMed:15543136, PubMed:28801535). Component of the NoRC-5 ISWI chromatin-remodeling complex (also called the NoRC chromatin-remodeling complex) at least composed of SMARCA5/SNF2H and BAZ2A/TIP5; the complex suppresses rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation (PubMed:28801535). Within the complex interacts with BAZ2A/TIP5 (PubMed:28801535). Within the complex interacts with HDAC1 (By similarity). Component of the BRF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and BAZ2B (PubMed:28801535). Within the complex interacts with BAZ2B (PubMed:28801535). Component of the NURF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and BPTF (PubMed:28801535). Within the complex interacts with BPFT (PubMed:28801535). Component of the CERF-5 ISWI chromatin-remodeling complex at least composed of SMARCA5/SNF2H and CECR2 (PubMed:28801535). LUZP1 is detected as part of the CERF-5 complex in embryonic stem cells where it is involved in complex stabilization but is not detected in the complex in the testis (By similarity). Within the complex interacts with CECR2 (PubMed:28801535). Component of the RSF-5 ISWI chromatin-remodeling complex (also called the RSF complex) at least composed of SMARCA5/SNF2H and RSF1 (PubMed:12972596, PubMed:28801535). Within the complex interacts with RSF1 (PubMed:12972596, PubMed:28801535). Interacts with the cohesin complex component RAD21; the interaction is direct (PubMed:12198550). Interacts with the NuRD complex components HDAC2, RBBP4 and CHD4; the interactions are direct (PubMed:12198550). Interacts with PCNA (PubMed:15543136). Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21 which positively regulates RNA polymerase III transcription (PubMed:16603771). Interacts with MYO1C (By similarity). Interacts with BEND3 (PubMed:26100909). Interacts with SIRT6; promoting recruitment to DNA damage sites (PubMed:23911928)
Research Field

Epigenetics and Nuclear Signaling
Synonyms
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Documentation
User Guide for Antibodies (1077 KB)

SMARCA5 Antibody (YA2013)(PBS only) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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