CHST6 Antibody
(Synonyms: Carbohydrate sulfotransferase 6, Corneal N-acetylglucosamine-6-O-sulfotransferase, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta, N-acetylglucosamine 6-O-sulfotransferase 5, C-GlcNAc6ST, hCGn6ST, GST4-beta, GlcNAc6ST-5, Gn6st-5, CHST6)CHST6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CHST6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 |
Product Details
CHST6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CHST6.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Synthetic peptide corresponding to the C-term region of human CHST6.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CHST6 is a Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Cooperates with B4GALT4 galactosyltransferase and B3GNT7 N-acetylglucosaminyltransferase to construct and elongate the sulfated disaccharide unit [->3Galbeta1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer. Involved in biosynthesis of keratan sulfate in cornea, with an impact on proteoglycan fibril organization and corneal transparency. Involved in sulfation of endothelial mucins such as GLYCAM1[1][2][3][4].
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Subcellular Localization
Golgi apparatus membrane
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Expression
Tissue_Specificity: Expressed in cornea. Mainly expressed in brain. Also expressed in spinal cord and trachea. -
Isoforms & Post-Translational Modification
CHST6 has an amino acid length of 395, molecular weight is 44099 Da.
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SwissProt ID
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Synonyms
Carbohydrate sulfotransferase 6, Corneal N-acetylglucosamine-6-O-sulfotransferase, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta, N-acetylglucosamine 6-O-sulfotransferase 5, C-GlcNAc6ST, hCGn6ST, GST4-beta, GlcNAc6ST-5, Gn6st-5, CHST6
Documentation
[1]. Akama TO, et al. Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal GlcNac 6-O-sulfotransferase both produce keratan sulfate. J Biol Chem. 2001 May 11;276(19):16271-8. [Content Brief]
[2]. Bartes A, et al. Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta). Biochem Biophys Res Commun. 2001 Apr 13;282(4):928-33. [Content Brief]
[3]. Akama TO, et al. Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate. J Biol Chem. 2002 Nov 8;277(45):42505-13. [Content Brief]
[4]. Kitayama K, et al. Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans. J Biol Chem. 2007 Oct 12;282(41):30085-96. [Content Brief]
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