Phospho-LRP6 (Ser1490) Antibody (YA10001)
(Synonyms: Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6)Phospho-LRP6 (Ser1490) Antibody (YA10001) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-LRP6 (Ser1490).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000-2000 |
Product Details
Phospho-LRP6 (Ser1490) Antibody (YA10001) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-LRP6 (Ser1490).
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 180 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 180 kDa
Synthetic phosphopeptide corresponding to residues surrounding S1490 of human LRP6.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
LRP6 is a Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in various processes including retinal angiogenesis and bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12].
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Subcellular Localization
Cell membrane; Endoplasmic reticulum; Membrane raft
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Expression
Tissue_Specificity: Widely coexpressed with LRP5 during embryogenesis and in adult tissues.
Induction: Decreased levels on WNT3A stimulation. -
Isoforms & Post-Translational Modification
Phospho-LRP6 has an amino acid length of 1613, molecular weight is 180429 Da.
Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479. -
Subunit
Homodimer; disulfide-linked.
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SwissProt ID
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Synonyms
Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6
Documentation
References
[1]. Mao B, et al. LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 2001 May 17;411(6835):321-5. [Content Brief]
[2]. Semënov MV, et al. Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6. Curr Biol. 2001 Jun 26;11(12):951-61. [Content Brief]
[3]. Li X, et al. Sclerostin binds to LRP5/6 and antagonizes canonical Wnt signaling. J Biol Chem. 2005 May 20;280(20):19883-7. [Content Brief]
[4]. Zeng X, et al. A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation. Nature. 2005 Dec 8;438(7069):873-7. [Content Brief]
[5]. Swiatek W, et al. Negative regulation of LRP6 function by casein kinase I epsilon phosphorylation. J Biol Chem. 2006 May 5;281(18):12233-41. [Content Brief]
[6]. Mi K, et al. Regulated proteolytic processing of LRP6 results in release of its intracellular domain. J Neurochem. 2007 Apr;101(2):517-29. [Content Brief]
[7]. Wei Q, et al. R-spondin1 is a high affinity ligand for LRP6 and induces LRP6 phosphorylation and beta-catenin signaling. J Biol Chem. 2007 May 25;282(21):15903-11. [Content Brief]
[8]. Piao S, et al. Direct inhibition of GSK3beta by the phosphorylated cytoplasmic domain of LRP6 in Wnt/beta-catenin signaling. PLoS One. 2008;3(12):e4046. [Content Brief]
[9]. Wu G, et al. Inhibition of GSK3 phosphorylation of beta-catenin via phosphorylated PPPSPXS motifs of Wnt coreceptor LRP6. PLoS One. 2009;4(3):e4926. [Content Brief]
[10]. Chen M, et al. G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway. J Biol Chem. 2009 Dec 11;284(50):35040-8. [Content Brief]
[11]. Berger BS, et al. Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6. EMBO Rep. 2017 May;18(5):712-725. [Content Brief]
[12]. Li S, et al. Variants in the Wnt co-receptor LRP6 are associated with familial exudative vitreoretinopathy. J Genet Genomics. 2022 Jun;49(6):590-594. [Content Brief]
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