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c-Myc Tag Antibody

Customer Review

Based on 1 Customer Validation

c-Myc Tag Antibody is a non-conjugated and Rabbt origined IgG polyclonal antibody, targeting to c-Myc-tag.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for c-Myc Tag Antibody
  • c-Myc Tag Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:10000 1:200

Product Details

Description

c-Myc Tag Antibody is a non-conjugated and Rabbt origined IgG polyclonal antibody, targeting to c-Myc-tag.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight NA
Immunogen

Synthetic peptide EQKLISEEDL coupled to KLH.

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for c-Myc Tag Antibody
    Western blot analysis of c-Myc was performed by loading 0.5 μg, 1 μg and 2 μg recombinant Myc-tagged Intelectin-1/ITLN1 protein onto a 4-20% GelHP Protein Gel (Tris-Gly). Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    c-Myc Tag is a The Myc tag is a short peptide tag derived from the c-Myc protein. It has no biological function itself, but it can be used for the detection, localization, purification, and interaction studies of target proteins.

c-Myc Tag Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of c-Myc was performed by loading 0.5 μg, 1 μg and 2 μg recombinant Myc-tagged Intelectin-1/ITLN1 protein onto a 4-20% GelHP Protein Gel (Tris-Gly). Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • c-Myc Tag Antibody Product Image
    1/16
  • Experimental Validation Results for c-Myc Tag Antibody
  • c-Myc Tag Antibody Product Image
  • Experimental Validation Results for c-Myc Tag Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of c-Myc was performed by loading 0.5 μg, 1 μg and 2 μg recombinant Myc-tagged Intelectin-1/ITLN1 protein onto a 4-20% GelHP Protein Gel (Tris-Gly). Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for c-Myc Tag Antibody
  • c-Myc Tag Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of c-Myc was performed by loading 0.5 μg, 1 μg and 2 μg recombinant Myc-tagged Intelectin-1/ITLN1 protein onto a 4-20% GelHP Protein Gel (Tris-Gly). Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • c-Myc Tag Antibody Product Image WB