CPEB4 Antibody
(Synonyms: KIAA1673, CPEB4, Cytoplasmic polyadenylation element-binding protein 4, CPE-BP4, CPE-binding protein 4, hCPEB-4)CPEB4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CPEB4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
CPEB4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CPEB4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 80 kDa
Synthetic peptide corresponding to the N-terminal region of human CPEB4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CPEB4 is a Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation. Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT. Stimulates proliferation of melanocytes. In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity)[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cell projection, dendrite; Cell projection, dendritic spine; Postsynaptic density; Cell projection, axon; Cell projection, growth cone; Endoplasmic reticulum; Cytoplasm, perinuclear region
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Expression
Tissue_Specificity: Expressed in pancreas in islets and ductal cells (at protein level). Expressed in melanocytes. -
Isoforms & Post-Translational Modification
CPEB4 has 3 isoforms, Q17RY0-1: amino acid length is 729, molecular weight is 80152 Da (predicted); Q17RY0-2: amino acid length is 712, molecular weight is 78314 Da (predicted); Q17RY0-3: amino acid length is 322, molecular weight is 36212 Da (predicted).CPEB4 存在 3 个异构体，Q17RY0-1：氨基酸个数为 729 个，分子量为 80152 Da (预测)；Q17RY0-2：氨基酸个数为 712 个，分子量为 78314 Da (预测)；Q17RY0-3：氨基酸个数为 322 个，分子量为 36212 Da (预测)。
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Subunit
Interacts with TOB1
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SwissProt ID
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Synonyms
KIAA1673, CPEB4, Cytoplasmic polyadenylation element-binding protein 4, CPE-BP4, CPE-binding protein 4, hCPEB-4
Documentation
[1]. Afroz T, et al. A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins. Genes Dev. 2014 Jul 1;28(13):1498-514. [Content Brief]
[2]. Giangarrà V, et al. Global Analysis of CPEBs Reveals Sequential and Non-Redundant Functions in Mitotic Cell Cycle. PLoS One. 2015;10(9):e0138794. [Content Brief]
[3]. Ortiz-Zapater E, et al. Key contribution of CPEB4-mediated translational control to cancer progression. Nat Med. 2011 Dec 4;18(1):83-90. [Content Brief]
[4]. Pérez-Guijarro E, et al. Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Nat Commun. 2016 Nov 18;7:13418. [Content Brief]
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