Osteopontin Antibody (YA9973)
(Synonyms: Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1)Based on 1 Customer Validation
Osteopontin Antibody (YA9973) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to Osteopontin Antibody.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-200 | 1:10-50 |
Product Details
Osteopontin Antibody (YA9973) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to Osteopontin Antibody.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Synthetic peptide corresponding to human Osteopontin.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、C2C12 (lane 3(20μg)) and C6 (lane 4(20μg)) using Osteopontin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P810729, 1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human liver tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded mouse bone tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded rat bone tissue using Osteopontin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P810729, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Background
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Function
Osteopontin is a Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction
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Subcellular Localization
Secreted
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Expression
Tissue_Specificity: Detected in cerebrospinal fluid and urine (at protein level). Bone. Found in plasma. -
Isoforms & Post-Translational Modification
Osteopontin has 4 isoforms, P10451-1: amino acid length is 314, molecular weight is 35423 Da (predicted); P10451-3: amino acid length is 287, molecular weight is 32355 Da (predicted); P10451-4: amino acid length is 292, molecular weight is 33017 Da (predicted); P10451-5: amino acid length is 300, molecular weight is 33843 Da (predicted).
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. -
Subunit
Interacts (via N-terminus) with integrin ITGA9:ITGB1
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SwissProt ID
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Synonyms
Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1
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