Lipoprotein Lipase Antibody (YA4954)

Cat. No.: HY-P85262: Data Sheet User Guide for Antibodies
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Lipoprotein Lipase Antibody (YA4954) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Lipoprotein Lipase.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Lipoprotein Lipase Antibody (YA4954) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Lipoprotein Lipase.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 53 kDa;

Observed band size: 53 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P06858

Gene ID

4023 [NCBI]

Immunogen

A synthesized peptide derived from human Lipoprotein lipase. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed:11342582, PubMed:27578112, PubMed:8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed:12032167, PubMed:7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed:24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed:11342582, PubMed:27811232)

Subcellular Localization：Cell membrane; Peripheral membrane protein; Extracellular side; Secreted; Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:Detected in plasma (PubMed:11893776, PubMed:12641539, PubMed:2340307) . Detected in milk (protein level) (PubMed:2340307) .

Subunit：Homodimer (Probable) (PubMed:11893776, PubMed:16179346, PubMed:26725083). Interacts with GPIHBP1 with 1:1 stoichiometry (PubMed:17997385, PubMed:26725083, PubMed:27811232, PubMed:27929370, PubMed:29899144, PubMed:30559189).

Synonyms

LPL; LIPD; Lipoprotein lipase; LPL

Documentation

User Guide for Antibodies (1077 KB)