MCM6 Antibody (YA7639)
(Synonyms: DNA replication licensing factor MCM6, p105MCM, MCM6)
Based on 1 Customer Validation
MCM6 Antibody (YA7639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF, IF-Tissue, IP, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:200-1000 | 1:200-1000 | 1:50-200 | 1:200-1000 | 1:20-50 | 1:50-100 |
Product Details
MCM6 Antibody (YA7639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.
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Host Rabbit
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Clonality Recombinant
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Species ReactivityHuman, Mouse, Rat Predicted Reactivity: Rat
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 93 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 93 kDa
A synthesized peptide derived from human MCM6: 650-821.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MCM6 acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity[1][2][3][4][5][6].
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Subcellular Localization
Nucleus,Chromosome
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Isoforms & Post-Translational Modification
Q14566: 821 amino acids, molecular weight 92889 Da.
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Subunit
Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731, PubMed:9305914)
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SwissProt ID
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Synonyms
DNA replication licensing factor MCM6, p105MCM, MCM6
Documentation
References
[1]. Tsuji T, et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell. 2006 Oct;17(10):4459-72. [Content Brief]
[2]. Rzechorzek NJ, et al. CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes. Nucleic Acids Res. 2020 Jul 9;48(12):6980-6995. [Content Brief]
[3]. Jones ML, et al. Structure of a human replisome shows the organisation and interactions of a DNA replication machine. EMBO J. 2021 Dec 1;40(23):e108819. [Content Brief]
[4]. Jenkyn-Bedford M, et al. A conserved mechanism for regulating replisome disassembly in eukaryotes. Nature. 2021 Dec;600(7890):743-747. [Content Brief]
[5]. Baris Y, et al. Fast and efficient DNA replication with purified human proteins. Nature. 2022 Jun;606(7912):204-210. [Content Brief]
[6]. Ishimi Y, et al. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 1997 Sep 26;272(39):24508-13. [Content Brief]
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