MCM6 Antibody (YA7639)

Cat. No.: HY-P87954: Data Sheet User Guide for Antibodies
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MCM6 Antibody (YA7639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

MCM6 Antibody (YA7639) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 93 kDa;

Observed band size: 93 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse Predicted Reactivity: Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q14566

Immunogen

A synthesized peptide derived from human MCM6

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425)

Subcellular Localization：Nucleus,Chromosome

Isoforms & Post-Translational Modification：Q14566: 821 amino acids, molecular weight 92889 Da.

Subunit：Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731, PubMed:9305914)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/u-937-cell.html [Content Brief]