ENSA Antibody (YA2770)(PBS only)

Cat. No.: HY-P83025A: Data Sheet User Guide for Antibodies
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ENSA Antibody (YA2770) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENSA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ENSA Antibody (YA2770) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENSA.

Host

Rabbit

Molecular Weight

Predicted band size: 13 kDa;

Observed band size: 13 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43768

Gene ID

2029 [NCBI]

Immunogen

A synthetic peptide of human ENSA

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
FC FC: Flow Cytometry	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas

Isoforms & Post-Translational Modification：O43768 has 9 isomers: O43768-1: 13389 Da (predicted); O43768-2: 12976 Da (predicted); O43768-3: 15281 Da (predicted); O43768-4: 15534 Da (predicted); O43768-5: 12818 Da (predicted); O43768-6: 12404 Da (predicted); O43768-7: 14710 Da (predicted); O43768-8: 11990 Da (predicted); O43768-9: 14868 Da (predicted).
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA

Subunit：Interacts (when phosphorylated at Ser-67) with PPP2R2D (By similarity). Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109。

Research Field

Neuroscience

Synonyms

ARPP-19e

Documentation

User Guide for Antibodies (1077 KB)