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FIP200 Antibody (YA761)

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Based on 1 Customer Validation

FIP200 Antibody (YA761) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FIP200.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FIP200 Antibody (YA761)
  • FIP200 Antibody (YA761) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
Predicted band size Info
 
Observed band size Info
 
Dilution Ratio 183 kDa 250/183 kDa

Product Details

Description

FIP200 Antibody (YA761) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FIP200.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 250/183 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 183 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human RB1CC1.AA range:651-800.

Sensitivity

Endogenous

Purification

Immunogen affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102313

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FIP200 Antibody (YA761)
    Western blot analysis of extracts from HEK293 (lane 2, 20ug), Raji (lane 3, 20ug), Hela (lane 4, 20ug) and MCF-7 (lane 5, 20ug) using FIP200 Antibody (HY-P80130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Involved in autophagy (PubMed:21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed:23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed:10769033, PubMed:12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed:12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed:14533007). Plays a crucial role in muscular differentiation (PubMed:12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity)

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, cytosol; Preautophagosomal structure; Lysosome

  • Expression


    Tissue_specificity:The expression level of this gene is closely related to the expression level of RB1 in cancer cell lines and various normal human tissues. It is highly expressed in human musculoskeletal cells and cultured osteosarcoma cells.

  • Subunit

    Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1 (PubMed:19211835, PubMed:19597335). This complex associates with ATG101 (PubMed:19211835, PubMed:19597335). Interacts with PTK2/FAK1 and PTK2B/PYK2 (PubMed:10769033, PubMed:12221124). Interacts with GABARAP and GABARAPL1 (PubMed:23043107). Interacts with ATG16L1; the interaction is required for ULK1 complex-dependent autophagy (PubMed:23262492, PubMed:23392225, PubMed:24954904, PubMed:28890335). Interacts with RNF111, SKI and SMAD7 (By similarity). Interacts with COP1 in the cytoplasm of proliferating cells in response to UV stimulation (PubMed:23289756). Interacts with TP53 (PubMed:21775823). Interacts with C9orf72 (PubMed:27334615). Interacts with WDR45B (PubMed:28561066). Interacts with ATG13; this interaction is increased in the absence of TMEM39A (PubMed:31806350). Interacts with WIPI2 (PubMed:28890335). Interacts with TAX1BP1 (PubMed:33226137, PubMed:34471133). Interacts (via phosphorylated FFAT motif) with MOSPD2, VAPA and VAPB (PubMed:33124732)

  • SwissProt ID

    Q8TDY2

  • Gene ID
    9821 [NCBI]
  • Research Field

    Signal Transduction

FIP200 Antibody (YA761) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2, 20ug), Raji (lane 3, 20ug), Hela (lane 4, 20ug) and MCF-7 (lane 5, 20ug) using FIP200 Antibody (HY-P80130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • FIP200 Antibody (YA761) Product Image
    1/16
  • Experimental Validation Results for FIP200 Antibody (YA761)
  • FIP200 Antibody (YA761) Product Image
  • Experimental Validation Results for FIP200 Antibody (YA761)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2, 20ug), Raji (lane 3, 20ug), Hela (lane 4, 20ug) and MCF-7 (lane 5, 20ug) using FIP200 Antibody (HY-P80130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FIP200 Antibody (YA761)
  • FIP200 Antibody (YA761) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2, 20ug), Raji (lane 3, 20ug), Hela (lane 4, 20ug) and MCF-7 (lane 5, 20ug) using FIP200 Antibody (HY-P80130). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • FIP200 Antibody (YA761) Product Image WB