VGluT1 Antibody (YA012)

Cat. No.: HY-P80957: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

VGluT1 Antibody (YA012) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VGluT1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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VGluT1 Antibody (YA012) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

VGluT1 Antibody (YA012) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VGluT1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 62 kDa;

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9P2U7

Gene ID

57030 [NCBI]

Immunogen

Synthetic peptide corresponding to Human VGluT1 aa500-560.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Neuro-2a(lane 2(20ug) and Neuro-2a(lane 3(40ug) using VGluT1 Antibody (HY-P80957) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Multifunctional transporter that transports L-glutamate as well as multiple ions such as cH2O2ide, proton, potassium, sodium and phosphate (PubMed:10820226). At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (By similarity). The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (By similarity). In addition, functions as a cH2O2ide channel that allows a cH2O2ide permeation tH2O2gh the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake (By similarity). The vesicular K(+)/H(+) antiport activity is electroneutral (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (PubMed:10820226). The symporter activity is driven by an inside negative membrane potential and is electrogenic (By similarity). Is necessary for synaptic signaling of visual-evoked responses from photoreceptors (By similarity)

Subcellular Localization：Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Cell membrane; Multi-pass membrane protein; Synapse, synaptosome

Expression：
Tissue_specificity:This gene is expressed in multiple regions of the brain, including the amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla oblongata, occipital lobe, putamen, and temporal lobe.

Subunit：Interacts with SHANK3

RRID

AB_3103006

Database

Entrez Gene: 57030 Human ; 72961 Mouse ; 116638 Rat

SwissProt: Q9P2U7 Human ; Q3TXX4 Mouse ; Q62634 Rat

OMIM: 605208 Human

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sw-620-cell.html [Content Brief]

VGluT1 Antibody (YA012) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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VGluT1 Antibody (YA012)

VGluT1 Antibody (YA012) Featured Recommendations:

Product Detail

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Background

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VGluT1 Antibody (YA012) Related Classifications

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