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DDX4 Antibody (YA4813)

(Synonyms: DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog)
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Based on 1 Customer Validation

DDX4 Antibody (YA4813) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDX4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DDX4 Antibody (YA4813)
  • DDX4 Antibody (YA4813) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

DDX4 Antibody (YA4813) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDX4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 79 kDa
Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DDX4 Antibody (YA4813)
    Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), U87 (lane 3), K562 (lane 4), 293T (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using DDX4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    DDX4 is an ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs[1][2].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:It is expressed only in the ovaries and testes. It is also expressed in migratory primordial germ cells in the gonadal crest region of both sexes.

  • Isoforms & Post-Translational Modification

    Q9NQI0 has 4 isomers: Q9NQI0-1: 79308 Da (predicted); Q9NQI0-2: 75821 Da (predicted); Q9NQI0-3: 63211 Da (predicted); Q9NQI0-4: 77056 Da (predicted).

  • Subunit

    Interacts with RANBP9 (PubMed:27622290). Interacts with RANBP10. Interacts with PIWIL2 and MAEL.

  • SwissProt ID

    Q9NQI0

  • Gene ID
    54514 [NCBI]
  • Synonyms

    DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog

DDX4 Antibody (YA4813) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), U87 (lane 3), K562 (lane 4), 293T (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using DDX4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • DDX4 Antibody (YA4813) Product Image
    1/16
  • Experimental Validation Results for DDX4 Antibody (YA4813)
  • DDX4 Antibody (YA4813) Product Image
  • Experimental Validation Results for DDX4 Antibody (YA4813)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), U87 (lane 3), K562 (lane 4), 293T (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using DDX4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for DDX4 Antibody (YA4813)
  • DDX4 Antibody (YA4813) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), U87 (lane 3), K562 (lane 4), 293T (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using DDX4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • DDX4 Antibody (YA4813) Product Image WB