DDX4 Antibody (YA4813)

Cat. No.: HY-P85121: Data Sheet COA
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DDX4 Antibody (YA4813) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDX4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

DDX4 Antibody (YA4813) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to DDX4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 79 kDa

Species Reactivity

Human

SwissProt ID

Q9NQI0

Gene ID

54514 [NCBI]

Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (30 μg) from HeLa (lane 2), U87 (lane 3), K562 (lane 4), 293T (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using DDX4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity)

Subcellular Localization：Cytoplasm; Cytoplasm, perinuclear region

Expression：
Tissue_specificity:It is expressed only in the ovaries and testes. It is also expressed in migratory primordial germ cells in the gonadal crest region of both sexes.

Isoforms & Post-Translational Modification：Q9NQI0 has 4 isomers: Q9NQI0-1: 79308 Da (predicted); Q9NQI0-2: 75821 Da (predicted); Q9NQI0-3: 63211 Da (predicted); Q9NQI0-4: 77056 Da (predicted).

Subunit：Interacts with RANBP9 (PubMed:27622290). Interacts with RANBP10. Interacts with PIWIL2 and MAEL.

Synonyms

DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)