Claudin 1 Antibody (YA5645)
(Synonyms: Claudin-1; Senescence-associated epithelial membrane protein; )
Based on 1 Customer Validation
Claudin 1 Antibody (YA5645) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Claudin 1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, WB, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1000 | 1:500-2000 | 1:100-500 | 1:1000-5000 |
Product Details
Claudin 1 Antibody (YA5645) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Claudin 1.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 22 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 22 kDa
Synthesized peptide derived from human Claudin 1 AA range: 150-211
Protein G
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HeLa(lane 2(20μg), A431(lane 3(20μg), HepG2(lane 4(20μg), HUVEC(lane 5(20μg) using Claudin 1 Antibody (HY-P85953). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
Claudin 1 is a Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions; (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus[1][2][3][4][5].
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Subcellular Localization
Cell junction, tight junction; Cell membrane; Multi-pass membrane protein; Basolateral cell membrane
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Expression
Tissue_specificity:It is highly expressed in the liver and kidneys. It is also expressed in the heart, brain, spleen, lungs, and testes. -
Subunit
Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members (PubMed:23704991).
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SwissProt ID
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Synonyms
Claudin-1; Senescence-associated epithelial membrane protein;
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Kirschner N, et al. Contribution of tight junction proteins to ion, macromolecule, and water barrier in keratinocytes. J Invest Dermatol. 2013 May;133(5):1161-9. [Content Brief]
[2]. Evans MJ, et al. Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry. Nature. 2007 Apr 12;446(7137):801-5. [Content Brief]
[3]. Harris HJ, et al. Claudin association with CD81 defines hepatitis C virus entry. J Biol Chem. 2010 Jul 2;285(27):21092-102. [Content Brief]
[4]. Douam F, et al. Critical interaction between E1 and E2 glycoproteins determines binding and fusion properties of hepatitis C virus during cell entry. Hepatology. 2014 Mar;59(3):776-88. [Content Brief]
[5]. Che P, et al. The interaction between claudin-1 and dengue viral prM/M protein for its entry. Virology. 2013 Nov;446(1-2):303-13. [Content Brief]
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