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RAMP1 Antibody (YA1818)(PBS only)

(Synonyms: CRLR activity-modifying protein 1; Calcitonin-receptor-like receptor activity-modifying protein 1)
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Based on 1 Customer Validation

RAMP1 Antibody (YA1818) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAMP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RAMP1 Antibody (YA1818)(PBS only)
  • RAMP1 Antibody (YA1818)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

RAMP1 Antibody (YA1818) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAMP1.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Immunogen

A synthetic peptide of human RAMP1 aa139-148.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RAMP1 Antibody (YA1818)(PBS only)
    Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using RAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    RAMP1 is an Accessory protein that interacts with and modulates the function of G-protein coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL) and calcitonin receptor (CALCR). Required for the transport of CALCRL to the plasma membrane. Together with CALCRL, form the receptor complex for the calcitonin gene-related peptides CGRP1/CALCA and CGRP2/CALCB. Together with CALCR, form the AMYR1 receptor complex for amylin/IAPP and CGRP1/CALCA[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract

  • Subunit

    Heterodimer of CALCRL and RAMP1; the interaction induces allosteric modulation of CALCRL function and CGRP1/CALCA and CGRP2/CALCB ligand specificity (PubMed:18725456, PubMed:20826335, PubMed:33602864). Heterodimer of CALCR and RAMP1; interaction forms the AMYR1 receptor complex for amylin/IAPP and CGRP1/CALCA ligands (PubMed:35324283, PubMed:38603770)

  • SwissProt ID

    O60894

  • Gene ID
    10267 [NCBI]
  • Synonyms

    CRLR activity-modifying protein 1; Calcitonin-receptor-like receptor activity-modifying protein 1

  • Research Field

    Neuroscience

References

[1]. Josephs TM, et al. Structure and dynamics of the CGRP receptor in apo and peptide-bound forms. Science. 2021 Apr 9;372(6538):.

[2]. McLatchie LM, et al. RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor. Nature. 1998 May 28;393(6683):333-9. [Content Brief]

[3]. Cao J, et al. A structural basis for amylin receptor phenotype. Science. 2022 Mar 25;375(6587):eabm9609. [Content Brief]

[4]. Cao J, et al. Cryo-EM Structure of the Human Amylin 1 Receptor in Complex with CGRP and Gs Protein. Biochemistry. 2024 May 7;63(9):1089-1096.

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RAMP1 Antibody (YA1818)(PBS only) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using RAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RAMP1 Antibody (YA1818)(PBS only) Product Image
    1/16
  • Experimental Validation Results for RAMP1 Antibody (YA1818)(PBS only)
  • RAMP1 Antibody (YA1818)(PBS only) Product Image
  • Experimental Validation Results for RAMP1 Antibody (YA1818)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using RAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for RAMP1 Antibody (YA1818)(PBS only)
  • RAMP1 Antibody (YA1818)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), NIH/3T3 (lane 3(20μg), C6 (lane 4(20μg), using RAMP1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • RAMP1 Antibody (YA1818)(PBS only) Product Image WB