1. Antibodies
  2. Primary Antibodies
  3. PKC beta 1 Antibody

PKC beta 1 Antibody

Cat. No.: HY-P80431
COA User Guide for Antibodies

PKC beta 1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 77 kDa, targeting to PKC beta 1. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
250 μL   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

PKC beta 1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 77 kDa, targeting to PKC beta 1. It can be used for WB,IHC-P,FC assays with tag free, in the background of Human, Mouse.

Tag

Free

Molecular Weight

Predicted band size: 77 kDa;Observed band size: 77 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm. Nucleus. Membrane.

Conjugation

Non-conjugated

Research Field

Signal Transduction

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse

SwissProt ID

SwissProt: P05771 Human;SwissProt: P68404 Mouse;SwissProt: P68403 Rat

Dilution Ratio

WB: 1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

  • Western blot analysis of extracts from C6(lane 2(10μg) or lane 3(20μg) ) and NIH3T3(lane 4(10μg) or lane 5(20μg) ) using PKC beta 1(HY-P80431) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80431, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB, IHC-P, FC

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Immunogen

Synthetic peptide corresponding to Human PKC beta 1.AA range:608-657.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PKC beta 1 Antibody
Cat. No.:
HY-P80431
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