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ATG7 Antibody (YA598)

(Synonyms: hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L)
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Based on 1 Customer Validation

ATG7 Antibody (YA598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATG7 Antibody (YA598)
  • ATG7 Antibody (YA598) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ATG7 Antibody (YA598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Apg7.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102612

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATG7 Antibody (YA598)
    Western blot analysis of extracts from M-brain (lane 1) and HepG2 (lane 2) using ATG7 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Atg7 is a core autophagy-related protein with E1-like enzyme activity, responsible for activating and transferring Atg8 (LC3/GABARAP) family proteins to facilitate autophagosome formation. In addition to its role in classical autophagy, Atg7 is involved in immune regulation (such as LC3-associated phagocytosis, LAP)， cell cycle control, apoptosis, and non-conventional protein secretion. It plays a crucial role in maintaining organelle homeostasis, removing damaged cellular components, and responding to cellular stress.
    Loss of Atg7 can lead to neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Moreover, Atg7 can both suppress tumor development and enhance tumor cell survival under extreme conditions. Atg7 also plays a significant role in infection defense and metabolic homeostasis, with its deficiency potentially increasing the risk of infections and being associated with metabolic diseases such as type 2 diabetes and fatty liver disease. Therefore, Atg7 is a potential research target for multiple diseases[1].

  • Subcellular Localization

    Cytoplasm; Preautophagosomal structure

  • Expression


    Tissue_specificity:It is widely expressed, especially in the kidneys, liver, lymph nodes, and bone marrow.

    Induction:Expression is up-regulated by the transcription factor HSF1

  • Isoforms & Post-Translational Modification

    O95352 has 3 isomers: O95352-1: 77960 Da (predicted); O95352-2: 75002 Da (predicted); O95352-3: 68618 Da (predicted).
    Acetylated by EP300;Polyubiquitinated on Lys-45 via 'Lys-63'-linked ubiquitin by TRIM32; this modification positiely regulates ATG8 and ATG12 activating enzyme activity leading to initiation of autophagy under metabolic stress

  • Subunit

    Homodimer (PubMed:11096062). Interacts with ATG3; this interaction is essential for the transfer of ATG8-like proteins's thioester from ATG7 to ATG3 and plays a role in the conjugation of ATG12 to ATG5 (PubMed:11825910, PubMed:26043688). Interacts with ATG12 (PubMed:11096062, PubMed:11825910). Forms intermediate conjugates with GABARAPL1 (By similarity). Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL2 or MAP1LC3A (PubMed:11096062, PubMed:16303767). Interacts with EP300 acetyltransferase (PubMed:19124466). Interacts with FOXO1 (PubMed:20543840)

  • SwissProt ID

    O95352

  • Gene ID
    10533 [NCBI]
  • Synonyms

    hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L

  • Research Field

    Cell Biology

[1]. Collier JJ, et al. Emerging roles of ATG7 in human health and disease. EMBO Mol Med. 2021 Dec 7;13(12):e14824. [Content Brief]

ATG7 Antibody (YA598) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from M-brain (lane 1) and HepG2 (lane 2) using ATG7 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • ATG7 Antibody (YA598) Product Image
    1/16
  • Experimental Validation Results for ATG7 Antibody (YA598)
  • ATG7 Antibody (YA598) Product Image
  • Experimental Validation Results for ATG7 Antibody (YA598)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from M-brain (lane 1) and HepG2 (lane 2) using ATG7 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for ATG7 Antibody (YA598)
  • ATG7 Antibody (YA598) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from M-brain (lane 1) and HepG2 (lane 2) using ATG7 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • ATG7 Antibody (YA598) Product Image WB