ATG7 Antibody (YA598)

Cat. No.: HY-P80559: Ficha de datos COA
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ATG7 Antibody (YA598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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ATG7 Antibody (YA598) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

ATG7 Antibody (YA598) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATG7.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 78 kDa;

Observed band size: 78 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O95352

Gene ID

10533 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Apg7.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from M-brain (lane 1) and HepG2 (lane 2) using ATG7 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes (PubMed:34161705). Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity)

Subcellular Localization：Cytoplasm; Preautophagosomal structure

Expression：
Tissue_specificity:It is widely expressed, especially in the kidneys, liver, lymph nodes, and bone marrow.

Induction:Expression is up-regulated by the transcription factor HSF1

Isoforms & Post-Translational Modification：O95352 has 3 isomers: O95352-1: 77960 Da (predicted); O95352-2: 75002 Da (predicted); O95352-3: 68618 Da (predicted).
Acetylated by EP300;Polyubiquitinated on Lys-45 via 'Lys-63'-linked ubiquitin by TRIM32; this modification positiely regulates ATG8 and ATG12 activating enzyme activity leading to initiation of autophagy under metabolic stress

Subunit：Homodimer (PubMed:11096062). Interacts with ATG3; this interaction is essential for the transfer of ATG8-like proteins's thioester from ATG7 to ATG3 and plays a role in the conjugation of ATG12 to ATG5 (PubMed:11825910, PubMed:26043688). Interacts with ATG12 (PubMed:11096062, PubMed:11825910). Forms intermediate conjugates with GABARAPL1 (By similarity). Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL2 or MAP1LC3A (PubMed:11096062, PubMed:16303767). Interacts with EP300 acetyltransferase (PubMed:19124466). Interacts with FOXO1 (PubMed:20543840)

RRID

AB_3102612

Database

Entrez Gene: 10533 Human ; 74244 Mouse ; 312647 Rat

SwissProt: O95352 Human ; Q9D906 Mouse ; Q641Y5 Rat

OMIM: 619422 Human

Research Field

Cell Biology

Synonyms

hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

ATG7 Antibody (YA598) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ATG7 Antibody (YA598)

ATG7 Antibody (YA598) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

ATG7 Antibody (YA598) Related Classifications

Productos vistos recientemente:

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