Integrin alpha 9 Antibody (YA1660)

Cat. No.: HY-P81915: Data Sheet COA
SDS
User Guide for Antibodies
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Integrin alpha 9 Antibody (YA1660) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Integrin alpha 9 Antibody (YA1660) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Integrin alpha 9 Antibody (YA1660) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin alpha 9.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 114 kDa;

Observed band size: 140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13797

Gene ID

3680 [NCBI]

Immunogen

A synthesized peptide derived from human Integrin alpha 9

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane 2(20μg), A549 (lane 3(20μg), NIH/3T3 (lane 4(20μg), C2C12 (lane 5(20μg) using Integrin alpha 9 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Integrin alpha-9/beta-1 (ITGA9:ITGB1) is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. ITGA9:ITGB1 may play a crucial role in SVEP1/polydom-mediated myoblast cell adhesion (By similarity). Integrin ITGA9:ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed:35802072)

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Expressed in vascular smooth muscle cells (at protein level) (PubMed:35802072) . Expressed in the airway epithelium (at protein level) (PubMed:8245132)

Subunit：Heterodimer of an alpha and a beta subunit. Alpha-9 (ITGA9) associates with beta-1 (ITGB1). Integrin ITGA9:ITGB1 interacts with FBLN5 (via N-terminus).

Database

Entrez Gene: 3680 Human ; 104099 Mouse ; 685004 Rat

SwissProt: Q13797 Human ;

OMIM: 603963 Human

Research Field

Signal Transduction

Synonyms

Alpha RLC; ITGA4L; ITGA9; RLC

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Integrin alpha 9 Antibody (YA1660) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Integrin alpha 9 Antibody (YA1660)

Integrin alpha 9 Antibody (YA1660) Featured Recommendations:

Product Detail

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Background

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Integrin alpha 9 Antibody (YA1660) Related Classifications

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