Topoisomerase II alpha Antibody (YA3759)
(Synonyms: TOP2; TP2A)Topoisomerase II alpha Antibody (YA3759) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Topoisomerase II alpha.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, ICC/IF, FC, ELISA
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Reactivity :
Human, Rat
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:100-1:500 | 1:200-1:400 | 1:10000 |
Product Details
Topoisomerase II alpha Antibody (YA3759) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Topoisomerase II alpha.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 174 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 174 kDa
Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Topoisomerase II alpha is key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation[1][2][3][4].
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Subcellular Localization
Cytoplasm; Nucleus, nucleoplasm; Nucleus; Nucleus, nucleolus
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Expression
Tissue_specificity:This gene is expressed in the tonsils, spleen, lymph nodes, thymus, skin, pancreas, testes, colon, kidneys, liver, brain, and lungs (PubMed:9155056) . It is also found in high-grade lymphoma, squamous cell carcinoma of the lung, and seminoma (PubMed:9155056) . -
Isoforms & Post-Translational Modification
P11388 has 4 isomers: P11388-1: 174385 Da (predicted); P11388-2: 177501 Da (predicted); P11388-3: 178712 Da (predicted); P11388-4: 182681 Da (predicted).
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation;(Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1; leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks;SUMOylated -
Subunit
Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity (PubMed:18790802, PubMed:20457750). Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity (PubMed:12711669). Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP (PubMed:23652018). Interacts with ERCC6 (PubMed:26030138). Interacts with PLSCR1 (PubMed:17567603). Interacts with GCNA; this interaction allows the resolution of topoisomerase II (TOP2A) DNA-protein cross-links (By similarity). Interacts with POL1RA/RPA1 (via dock II) and UBTF in the context of Pol I complex; may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)
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SwissProt ID
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Synonyms
TOP2; TP2A
Documentation
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