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MSH2 Antibody (YA1774)

(Synonyms: DNA mismatch repair protein Msh2; MutS protein homolog 2)
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Based on 1 Customer Validation

MSH2 Antibody (YA1774) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MSH2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MSH2 Antibody (YA1774)
  • MSH2 Antibody (YA1774) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MSH2 Antibody (YA1774) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MSH2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 105 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 105 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human MSH2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104150

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MSH2 Antibody (YA1774)
    Western blot analysis of extracts from Hela (lane 2, 20ug), HepG2 (lane 3, 20ug), HL-60 (lane 4, 20ug) and A375 (lane 5, 20ug) using MSH2 Antibody (HY-P82029). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    MSH2 component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis[1].

  • Subcellular Localization

    Nucleus; Chromosome

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    P43246 has 2 isomers: P43246-1: 104743 Da (predicted); P43246-2: 97323 Da (predicted).
    Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway;Sequentially deacetylated and polyubiquitinated by HDAC6, leading to MSH2 degradation

  • Subunit

    Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1 (PubMed:26300262). Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2-MSH3 (MutS beta) (PubMed:8942985). Both heterodimers form a ternary complex with MutL alpha (MLH1-PMS1) (PubMed:10856833, PubMed:11427529, PubMed:11429708, PubMed:12414623, PubMed:14676842, PubMed:9788596). Interacts with MCM9; the interaction recruits MCM9 to chromatin (PubMed:26300262). Interacts with MCM8 (PubMed:26300262). Interacts with EXO1 (PubMed:10856833, PubMed:11427529, PubMed:11429708, PubMed:12414623, PubMed:14676842, PubMed:9788596). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex (PubMed:10783165). This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains (PubMed:10783165). Interacts with ATR (PubMed:14657349). Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases (PubMed:19596235). Interacts with SMARCAD1 (PubMed:18675275)

  • SwissProt ID

    P43246

  • Gene ID
    4436 [NCBI]
  • Synonyms

    DNA mismatch repair protein Msh2; MutS protein homolog 2

  • Research Field

    Epigenetics and Nuclear Signaling

MSH2 Antibody (YA1774) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), HepG2 (lane 3, 20ug), HL-60 (lane 4, 20ug) and A375 (lane 5, 20ug) using MSH2 Antibody (HY-P82029). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MSH2 Antibody (YA1774) Product Image
    1/16
  • Experimental Validation Results for MSH2 Antibody (YA1774)
  • MSH2 Antibody (YA1774) Product Image
  • Experimental Validation Results for MSH2 Antibody (YA1774)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), HepG2 (lane 3, 20ug), HL-60 (lane 4, 20ug) and A375 (lane 5, 20ug) using MSH2 Antibody (HY-P82029). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MSH2 Antibody (YA1774)
  • MSH2 Antibody (YA1774) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug), HepG2 (lane 3, 20ug), HL-60 (lane 4, 20ug) and A375 (lane 5, 20ug) using MSH2 Antibody (HY-P82029). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MSH2 Antibody (YA1774) Product Image WB