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KLC1 Antibody (YA2363)

(Synonyms: Kinesin2; KLC; Klc1; KNS2; KNS2A)
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Based on 1 Customer Validation

KLC1 Antibody (YA2363) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KLC1 Antibody (YA2363)
  • KLC1 Antibody (YA2363) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100 1:50

Product Details

Description

KLC1 Antibody (YA2363) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KLC1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KLC1 aa501-573.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KLC1 Antibody (YA2363)
    Western blot analysis of extracts from Hela (lane2, 20μg), Rat brain tissue (lane3, 20μg) and MCF-7 (lane4, 20μg) using KLC1 Antibody (HY-P82618). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    KLC1 is a Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity[1].

  • Subcellular Localization

    Cell projection, growth cone; Cytoplasmic vesicle; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Found in a variety of tissues. Mostly abundant in brain and spine

  • Isoforms & Post-Translational Modification

    Q07866 has 10 isomers: Q07866-1: 65310 Da (predicted); Q07866-2: 63816 Da (predicted); Q07866-3: 64319 Da (predicted); Q07866-4: 71996 Da (predicted); Q07866-5: 69967 Da (predicted); Q07866-6: 71006 Da (predicted); Q07866-7: 66292 Da (predicted); Q07866-8: 62826 Da (predicted); Q07866-9: 72398 Da (predicted); Q07866-10: 69765 Da (predicted).
    Phosphorylation at Ser-460 by ERK inhibits interaction with CLSTN1 and localization to cytoplasmic vesicles

  • Subunit

    Oligomeric complex composed of two heavy chains and two light chains. Interacts (via TPR repeats) with TOR1A; the interaction associates TOR1A with the kinesin oligomeric complex (PubMed:14970196).

  • SwissProt ID

    Q07866

  • Gene ID
    3831 [NCBI]
  • Synonyms

    Kinesin2; KLC; Klc1; KNS2; KNS2A

  • Research Field

    Signal Transduction

KLC1 Antibody (YA2363) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Rat brain tissue (lane3, 20μg) and MCF-7 (lane4, 20μg) using KLC1 Antibody (HY-P82618). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • KLC1 Antibody (YA2363) Product Image
    1/16
  • Experimental Validation Results for KLC1 Antibody (YA2363)
  • KLC1 Antibody (YA2363) Product Image
  • Experimental Validation Results for KLC1 Antibody (YA2363)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), Rat brain tissue (lane3, 20μg) and MCF-7 (lane4, 20μg) using KLC1 Antibody (HY-P82618). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KLC1 Antibody (YA2363)
  • KLC1 Antibody (YA2363) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), Rat brain tissue (lane3, 20μg) and MCF-7 (lane4, 20μg) using KLC1 Antibody (HY-P82618). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • KLC1 Antibody (YA2363) Product Image WB