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eIF4EBP3 Antibody (YA1080)(PBS only)

(Synonyms: 4EBP3; 4E-BP3)

eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for eIF4EBP3 Antibody (YA1080)(PBS only)
  • eIF4EBP3 Antibody (YA1080)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 11 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Immunogen

Recombinant protein of human EIF4EBP3.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for eIF4EBP3 Antibody (YA1080)(PBS only)
    Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), CHO-K1(EGFP-EIF4EBP3) (lane 2, 15 μg) using 4E BP3 (3B6) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    eIF4EBP3 is a Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: the hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repression of translation. In contrast, the hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Inhibits EIF4E-mediated mRNA nuclear export[1].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:The gene is expressed at the highest levels in skeletal muscle, heart, kidneys, and pancreas, but at very low levels in the brain and thymus.

  • Subunit

    Interacts with EIF4E (PubMed:9593750). Interacts with RPA2 (in unphosphorylated form via N-terminus); the interaction enhances EIF4EBP3-mediated inhibition of EIF4E-mediated mRNA nuclear export (PubMed:22684010)

  • SwissProt ID

    O60516

  • Gene ID
    8637 [NCBI]
  • Synonyms

    4EBP3; 4E-BP3

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Chen CC, et al. 4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2. FEBS Lett. 2012 Jul 30;586(16):2260-6. [Content Brief]

eIF4EBP3 Antibody (YA1080)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), CHO-K1(EGFP-EIF4EBP3) (lane 2, 15 μg) using 4E BP3 (3B6) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • eIF4EBP3 Antibody (YA1080)(PBS only) Product Image
    1/16
  • Experimental Validation Results for eIF4EBP3 Antibody (YA1080)(PBS only)
  • eIF4EBP3 Antibody (YA1080)(PBS only) Product Image
  • Experimental Validation Results for eIF4EBP3 Antibody (YA1080)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), CHO-K1(EGFP-EIF4EBP3) (lane 2, 15 μg) using 4E BP3 (3B6) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for eIF4EBP3 Antibody (YA1080)(PBS only)
  • eIF4EBP3 Antibody (YA1080)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from CHO-K1 (lane 1, 15 μg), CHO-K1(EGFP-EIF4EBP3) (lane 2, 15 μg) using 4E BP3 (3B6) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • eIF4EBP3 Antibody (YA1080)(PBS only) Product Image WB