eIF4EBP3 Antibody (YA1080)(PBS only)

Cat. No.: HY-P81335A: Data Sheet User Guide for Antibodies
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eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

eIF4EBP3 Antibody (YA1080) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4EBP3.

Host

Mouse

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: 11 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60516

Gene ID

8637 [NCBI]

Immunogen

Recombinant protein of human EIF4EBP3.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: the hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repression of translation. In contrast, the hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed:22684010)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:The gene is expressed at the highest levels in skeletal muscle, heart, kidneys, and pancreas, but at very low levels in the brain and thymus.

Subunit：Interacts with EIF4E (PubMed:9593750). Interacts with RPA2 (in unphosphorylated form via N-terminus); the interaction enhances EIF4EBP3-mediated inhibition of EIF4E-mediated mRNA nuclear export (PubMed:22684010)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

4EBP3; 4E-BP3

Documentation

User Guide for Antibodies (1077 KB)